Goat Anti-Rabbit IgG H&L (Biotin) (ab97049)
Key features and details
- Goat Anti-Rabbit IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, ELISA, WB
Overview
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Product name
Goat Anti-Rabbit IgG H&L (Biotin)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody reactivity was detected against non-immunoglobulin serum proteins.
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Tested applications
Suitable for: IHC-P, ICC/IF, ELISA, WBmore details -
Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells. -
Conjugation notes
Biotin/ antibody protein ratio is 4:1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/500 - 1/5000.
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ICC/IF |
1/200 - 1/1000.
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ELISA |
1/20000 - 1/400000. ELISA-Primary
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WB |
1/20000 - 1/400000. WB-Chemiluminescent
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Notes |
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IHC-P
1/500 - 1/5000. |
ICC/IF
1/200 - 1/1000. |
ELISA
1/20000 - 1/400000. ELISA-Primary |
WB
1/20000 - 1/400000. WB-Chemiluminescent |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (ab97049)
Ab97049 was used at dilution 1:500 for IHC analysis of GATA4 expression in paraformaldehyde fixed, paraffin embedded testis sections. The primary antibody was a rabbit polyclonal IgG raised against human GATA4.
Image on the left: isotype control image
This image is courtesy of Dr Kyle Caires
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (14)
ab97049 has been referenced in 14 publications.
- Ding H et al. Upregulation of miR-101a Suppresses Chronic Renal Fibrosis by Regulating KDM3A via Blockade of the YAP-TGF-ß-Smad Signaling Pathway. Mol Ther Nucleic Acids 19:1276-1289 (2020). PubMed: 32092824
- Li Q et al. Knockdown of KLK12 inhibits viability and induces apoptosis in human colorectal cancer HT-29 cell line. Int J Mol Med 44:1667-1676 (2019). PubMed: 31485623
- Wu XS et al. Tocilizumab promotes corneal allograft survival in rats by modulating Treg-Th17 balance. Int J Ophthalmol 12:1823-1831 (2019). PubMed: 31850163
- Pacini V et al. Identification of a pituitary ERa-activated enhancer triggering the expression of Nr5a1, the earliest gonadotrope lineage-specific transcription factor. Epigenetics Chromatin 12:48 (2019). PubMed: 31391075
- Van Den Berge N et al. Evidence for bidirectional and trans-synaptic parasympathetic and sympathetic propagation of alpha-synuclein in rats. Acta Neuropathol 138:535-550 (2019). PubMed: 31254094