Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin)
  • Host species
    Goat
  • Target species
    Rabbit
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
  • Conjugation notes
    Biotin/ antibody protein ratio is 4:1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/5000.
ICC/IF 1/200 - 1/1000.
ELISA 1/20000 - 1/400000. ELISA-Primary
WB 1/20000 - 1/400000. WB-Chemiluminescent

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) (ab97049)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) (ab97049)

    Ab97049 was used at dilution 1:500 for IHC analysis of GATA4 expression in paraformaldehyde fixed, paraffin embedded testis sections. The primary antibody was a rabbit polyclonal IgG raised against human GATA4.

    Image on the left: isotype control image

    This image is courtesy of Dr Kyle Caires

References

This product has been referenced in:
  • Siwowska K  et al. Folate Receptor-Positive Gynecological Cancer Cells: In Vitro and In Vivo Characterization. Pharmaceuticals (Basel) 10:N/A (2017). Read more (PubMed: 28809784) »
  • Zhao H  et al. Expression of decorin in intestinal tissues of mice with inflammatory bowel disease and its correlation with autophagy. Exp Ther Med 12:3885-3892 (2016). IHC . Read more (PubMed: 28105121) »

See all 5 Publications for this product

Publishing research using ab97049? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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8 reviews or Q&A

10
Votes: Highest First

Ki-67 staining in mouse prostate tissue using Abcam secondary antibody ab97049

 Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Ki-67 staining in mouse prostate tissue using Abcam secondary antibody Goat anti-Rabbit IgG H&L (Biotin) (ab97049)
abreview image
Username

Abcam user community

Verified customer

Submitted Apr 08 2015

Hi Abcam,

Do you mind helping with this enquiry below?

We have found another couple of secondary antibodies on your website that appear identical to AB6788 & AB6720 except for a different concentration.

They are: ab...

Read More

Thank you for contacting us and you interest in our products.

There are a few differences between the two pairs of antibodies you have identified. A few of these include the purification used (affitiny vs IgG fraction) as well as the ratio of...

Read More

Fluorescent detection with biotinylated secondary antibody is not giving strong signal. Would like to try avidin-biotin complex staining with DAB to amplify signal.

Thank you so much for your call today and for letting us know about the trouble with the staining.

As we discussed, I'm sending a free of charge DAB avidin-biotin complex staining kit, ab64261, which should arrive on Monday on the order ***. ...

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J'ai commandé un anticorps anti cd31 ab28364 pour faire de l'immunohistochimie ainsi qu'un anticorps secondaire ab97049.

J'aimerais savoir quel kit de révélation vous me conseiller pour révéler ce marquage ...

Read More

Merci de nous avoir contactés.

Les deux kits que je vous recommende sont :

Streptavidin Alkaline Phosphatase (Ready to Use), www.abcam.com/ab64268
Streptavidin Peroxidase (Ready to Use), www.abcam.com/ab64269

...

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HI

I NEED CERTIFICATE OF ANALYSIS OF THE FOLLOWING PRODUCTS:

CAT. NUMBER LOT

AB27969 GR66929-1

AB1793 GR66247-1

AB2105 GR24376-3

AB6672 GR58964-2

AB34843 GR32935-5

...

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Thank you for contacting Abcam.

Please find attached the requested Certificate of Compliances.

If there is anything else I can help you, please let me know.

dear colleagues
hallo
can i ask about the optimal dilution of these products.
1-ab33923
2-ab6668
3-ab6822
4-ab7481
5-ab97049

Thank you for contacting Abcam.

As with most antibodies the dilution that works best for you depends on multiple varaibles and so you should do some titrations to find the best for you, but I placed a good starting point next to each.
Read More

Dear Technical Team, Hope this e-mail finds you well! Kindly arrange to send us the certificate of compliance (CoC) for the below Abcam products as desired by one of our customers : Cat No. Description ab97021 Goat polyclonal Secondary Antibo...

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I am beginning to gather the CoC's you have requested. When you have a chance, please provide the lot number from each catalog number so I can include this information on the documentation. Thanks for the additional information.

Dear Abcam:   How many  test for IHQ-P can the client realized with these antibodies? I understand that it depend of the dilution, but aprx.   AB27969 AB1793 AB2105 AB6672 AB34843 AB97049 AB97263   I have problems wi...

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Thank you for your email! The best way to figure out dilutions and number of tests is to look at the datasheet. In most cases we will have a recommended dilution for each application, and will also include the concentration of the product along...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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