Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin)
  • Host species
    Goat
  • Target species
    Rabbit
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
  • Conjugation notes
    Biotin/ antibody protein ratio is 4:1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/5000.
ICC/IF 1/200 - 1/1000.
ELISA 1/20000 - 1/400000. ELISA-Primary
WB 1/20000 - 1/400000. WB-Chemiluminescent

Images

  • Ab97049 was used at dilution 1:500 for IHC analysis of GATA4 expression in paraformaldehyde fixed, paraffin embedded testis sections. The primary antibody was a rabbit polyclonal IgG raised against human GATA4.

    Image on the left: isotype control image

    This image is courtesy of Dr Kyle Caires

References

This product has been referenced in:
  • Donfack NJ  et al. Stroma cell-derived factor 1 and connexins (37 and 43) are preserved after vitrification and in vitro culture of goat ovarian cortex. Theriogenology 116:83-88 (2018). Read more (PubMed: 29783047) »
  • Johnson ZI  et al. TNF-a promotes nuclear enrichment of the transcription factor TonEBP/NFAT5 to selectively control inflammatory but not osmoregulatory responses in nucleus pulposus cells. J Biol Chem 292:17561-17575 (2017). Read more (PubMed: 28842479) »
See all 7 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Ki-67 staining in mouse prostate tissue using Abcam secondary antibody Goat anti-Rabbit IgG H&L (Biotin) (ab97049)

Abcam user community

Verified customer

Submitted Apr 08 2015

Answer

Thank you for contacting us and you interest in our products.

There are a few differences between the two pairs of antibodies you have identified. A few of these include the purification used (affitiny vs IgG fraction) as well as the ratio of conjugation. The ab6788 and ab6720 is estimated to have a conjugation ratio of 10-20 per IgG molecule whilst ab97049 and ab97021 have a ratio of 1:4.

I am not familiar with the amplification technique you intend to use and cannot therefore anticipate how these differences may alter the results.

If you could share a more detailed description of the procedure I may be able to advise in more detail.

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

Read More

Answer

Thank you so much for your call today and for letting us know about the trouble with the staining.

As we discussed, I'm sending a free of charge DAB avidin-biotin complex staining kit, ab64261, which should arrive on Monday on the order ***.

Please keep me updated about the results after using the kit, and let me know if you have any questions or if there is anything else that we can do for you.

Have a wonderful weekend!

Read More

Answer

Merci de nous avoir contactés.

Les deux kits que je vous recommende sont :

Streptavidin Alkaline Phosphatase (Ready to Use), www.abcam.com/ab64268
Streptavidin Peroxidase (Ready to Use), www.abcam.com/ab64269



Je vous fais parvenir un devis dans un autre email.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Answer

Thank you for contacting Abcam.

Please find attached the requested Certificate of Compliances.

If there is anything else I can help you, please let me know.

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Answer

Thank you for contacting Abcam.

As with most antibodies the dilution that works best for you depends on multiple varaibles and so you should do some titrations to find the best for you, but I placed a good starting point next to each.

1-ab33923 - IHC-P: 1/50
2-ab6668 - ICC/IF: 1/1000, WB: 1/2000, ELISA: 1/50000
3-ab6822 - WB: 1/2000, ELISA: 1/200000, ICC/IF: 1/1000, IHC-P:1/100
4-ab7481 -10%
5-ab97049 - IHC-P: 1/500, ICC/IF: 1/200, ELISA: 1/20000, WB: 1/10000.

Ifthere is anythingelse I can help you with, or ifyou would like a starting concentration for an application that I did not list about, then please let me know.

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Answer

Thank you for your email! The best way to figure out dilutions and number of tests is to look at the datasheet. In most cases we will have a recommended dilution for each application, and will also include the concentration of the product along with the amount of product in the vial. Using these pieces of information, you can get the number of experiments that you will be able to do with that product. In order to give you the number of tests, I will need to know what application you are looking to use the antibodies in. The calculator at the following link may help you: https://www.msu.edu/~nixonjos/more/dilution.html?stock=1&needed=0.0001&volume=1&total=0.1 To get the number of tests, you can divide the final result ("total stock solution to use") by the amount included in the tube. I hope this helps. If you have any further questions, please do not hesitat

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