Goat Anti-Rabbit IgG H&L (Biotin) (ab97049)

Reviews (1)Q&A (7)References (9)

Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (Biotin)
    See all IgG secondary antibodies

  • Description

    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin)

  • Host species

    Goat

  • Target species

    Rabbit

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

  • Tested applications

    Suitable for: IHC-P, ICC/IF, ELISA, WBmore details

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.

  • Conjugation notes

    Biotin/ antibody protein ratio is 4:1

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/5000.
ICC/IF 1/200 - 1/1000.
ELISA 1/20000 - 1/400000. ELISA-Primary
WB 1/20000 - 1/400000. WB-Chemiluminescent

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (ab97049)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (ab97049)

    Ab97049 was used at dilution 1:500 for IHC analysis of GATA4 expression in paraformaldehyde fixed, paraffin embedded testis sections. The primary antibody was a rabbit polyclonal IgG raised against human GATA4.

    Image on the left: isotype control image

    This image is courtesy of Dr Kyle Caires

References

This product has been referenced in:

  • Pacini V  et al. Identification of a pituitary ERa-activated enhancer triggering the expression of Nr5a1, the earliest gonadotrope lineage-specific transcription factor. Epigenetics Chromatin 12:48 (2019). Read more (PubMed: 31391075) »
  • Van Den Berge N  et al. Evidence for bidirectional and trans-synaptic parasympathetic and sympathetic propagation of alpha-synuclein in rats. Acta Neuropathol 138:535-550 (2019). Read more (PubMed: 31254094) »
See all 9 Publications for this product

Publishing research using ab97049? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-8 of 8 Abreviews or Q&A

Ki-67 staining in mouse prostate tissue using Abcam secondary antibody ab97049

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Ki-67 staining in mouse prostate tissue using Abcam secondary antibody Goat anti-Rabbit IgG H&L (Biotin) (ab97049)

Abcam user community

Verified customer

Submitted Apr 08 2015

Question

Hi Abcam,

Do you mind helping with this enquiry below?

We have found another couple of secondary antibodies on your website that appear identical to AB6788 & AB6720 except for a different concentration.

They are: ab97049 Goat anti-rabbit IgG H&L (biotin)

and ab 97021 Goat polyclonal Secondary antibody to mouse IgG- H&L (Biotin)

What we are hoping to do is to enhance a western blot signal by adding a streptavidin step prior to applying a biotinylated secondary antibody. (This has been successfully done by colleagues in the past).

Would it be possible to get some advice as to which ones would be more suitable for our application?

Thanks for your help :D

Kind regards,

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Answer

Thank you for contacting us and you interest in our products.

There are a few differences between the two pairs of antibodies you have identified. A few of these include the purification used (affitiny vs IgG fraction) as well as the ratio of conjugation. The ab6788 and ab6720 is estimated to have a conjugation ratio of 10-20 per IgG molecule whilst ab97049 and ab97021 have a ratio of 1:4.

I am not familiar with the amplification technique you intend to use and cannot therefore anticipate how these differences may alter the results.

If you could share a more detailed description of the procedure I may be able to advise in more detail.

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

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Question

Fluorescent detection with biotinylated secondary antibody is not giving strong signal. Would like to try avidin-biotin complex staining with DAB to amplify signal.

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Answer

Thank you so much for your call today and for letting us know about the trouble with the staining.

As we discussed, I'm sending a free of charge DAB avidin-biotin complex staining kit, ab64261, which should arrive on Monday on the order ***.

Please keep me updated about the results after using the kit, and let me know if you have any questions or if there is anything else that we can do for you.

Have a wonderful weekend!

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Question

J'ai commandé un anticorps anti cd31 ab28364 pour faire de l'immunohistochimie ainsi qu'un anticorps secondaire ab97049.

J'aimerais savoir quel kit de révélation vous me conseiller pour révéler ce marquage en immuno histo

et est ce que par la même occasion vous pouvez me faire parvenir un devis, pour les kits concernés.

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Answer

Merci de nous avoir contactés.

Les deux kits que je vous recommende sont :

Streptavidin Alkaline Phosphatase (Ready to Use), www.abcam.com/ab64268
Streptavidin Peroxidase (Ready to Use), www.abcam.com/ab64269



Je vous fais parvenir un devis dans un autre email.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Question

HI

I NEED CERTIFICATE OF ANALYSIS OF THE FOLLOWING PRODUCTS:

CAT. NUMBER LOT

AB27969 GR66929-1

AB1793 GR66247-1

AB2105 GR24376-3

AB6672 GR58964-2

AB34843 GR32935-5

AB97049 GR46176-1

THANKS

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Answer

Thank you for contacting Abcam.

Please find attached the requested Certificate of Compliances.

If there is anything else I can help you, please let me know.

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Question

dear colleagues
hallo
can i ask about the optimal dilution of these products.
1-ab33923
2-ab6668
3-ab6822
4-ab7481
5-ab97049

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Answer

Thank you for contacting Abcam.

As with most antibodies the dilution that works best for you depends on multiple varaibles and so you should do some titrations to find the best for you, but I placed a good starting point next to each.

1-ab33923 - IHC-P: 1/50
2-ab6668 - ICC/IF: 1/1000, WB: 1/2000, ELISA: 1/50000
3-ab6822 - WB: 1/2000, ELISA: 1/200000, ICC/IF: 1/1000, IHC-P:1/100
4-ab7481 -10%
5-ab97049 - IHC-P: 1/500, ICC/IF: 1/200, ELISA: 1/20000, WB: 1/10000.

Ifthere is anythingelse I can help you with, or ifyou would like a starting concentration for an application that I did not list about, then please let me know.

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Question

Dear Technical Team, Hope this e-mail finds you well! Kindly arrange to send us the certificate of compliance (CoC) for the below Abcam products as desired by one of our customers : Cat No. Description ab97021 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) ab97049 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) ab97023 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) ab60314 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) ab97080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed ab65623 ECL Western Blotting Substrate Kit - 50 Tests ab52476 Rabbit monoclonal [EP1829Y] to CaMKII ab11800 Goat polyclonal to DCXR ab7260 Rabbit polyclonal to GFAP - Astrocyte Marker ab104224 Mouse monoclonal [1B7] to FOX3 ab76260 Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9) ab64230 Mounting Medium ab82477 Rabbit polyclonal to Adenosine A1 Receptor ab3462 Rabbit polyclonal to alpha 1 Adrenergic Receptor

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Answer

I am beginning to gather the CoC's you have requested. When you have a chance, please provide the lot number from each catalog number so I can include this information on the documentation. Thanks for the additional information.

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Question

Dear Abcam:   How many  test for IHQ-P can the client realized with these antibodies? I understand that it depend of the dilution, but aprx.   AB27969 AB1793 AB2105 AB6672 AB34843 AB97049 AB97263   I have problems with dilutions, I would like learn about it. Do you have some examples o documents for it?   Thanks and B

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Answer

Thank you for your email! The best way to figure out dilutions and number of tests is to look at the datasheet. In most cases we will have a recommended dilution for each application, and will also include the concentration of the product along with the amount of product in the vial. Using these pieces of information, you can get the number of experiments that you will be able to do with that product. In order to give you the number of tests, I will need to know what application you are looking to use the antibodies in. The calculator at the following link may help you: https://www.msu.edu/~nixonjos/more/dilution.html?stock=1&needed=0.0001&volume=1&total=0.1 To get the number of tests, you can divide the final result ("total stock solution to use") by the amount included in the tube. I hope this helps. If you have any further questions, please do not hesitat

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