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  1. Link

    goat-rabbit-igg-hl-biotin-ab97049.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rabbit IgG H&L (Biotin) (ab97049)

  • Datasheet
  • SDS
Reviews (1)Q&A (7)References (19)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (ab97049)

    Key features and details

    • Goat Anti-Rabbit IgG H&L (Biotin)
    • Conjugation: Biotin
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: IHC-P, ICC/IF, ELISA, WB

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    Overview

    • Product name

      Goat Anti-Rabbit IgG H&L (Biotin)
      See all IgG secondary antibodies
    • Host species

      Goat
    • Target species

      Rabbit
    • Specificity

      By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody reactivity was detected against non-immunoglobulin serum proteins.

    • Tested applications

      Suitable for: IHC-P, ICC/IF, ELISA, WBmore details
    • Conjugation

      Biotin

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
    • Conjugation notes

      Biotin/ antibody protein ratio is 4:1
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Rabbit
      • IgG
      • Biotin

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab97049 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P (1)
    1/500 - 1/5000.
    ICC/IF
    1/200 - 1/1000.
    ELISA
    1/20000 - 1/400000. ELISA-Primary
    WB
    1/20000 - 1/400000. WB-Chemiluminescent
    Notes
    IHC-P
    1/500 - 1/5000.
    ICC/IF
    1/200 - 1/1000.
    ELISA
    1/20000 - 1/400000. ELISA-Primary
    WB
    1/20000 - 1/400000. WB-Chemiluminescent

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (ab97049)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (ab97049)

      Ab97049 was used at dilution 1:500 for IHC analysis of GATA4 expression in paraformaldehyde fixed, paraffin embedded testis sections. The primary antibody was a rabbit polyclonal IgG raised against human GATA4.

      Image on the left: isotype control image

      This image is courtesy of Dr Kyle Caires

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (19)

    Publishing research using ab97049? Please let us know so that we can cite the reference in this datasheet.

    ab97049 has been referenced in 19 publications.

    • Ding H  et al. Upregulation of miR-101a Suppresses Chronic Renal Fibrosis by Regulating KDM3A via Blockade of the YAP-TGF-ß-Smad Signaling Pathway. Mol Ther Nucleic Acids 19:1276-1289 (2020). PubMed: 32092824
    • Harding CF  et al. Mold inhalation causes innate immune activation, neural, cognitive and emotional dysfunction. Brain Behav Immun 87:218-228 (2020). PubMed: 31751617
    • Allen A  et al. Actin-binding protein profilin1 promotes aggressiveness of clear-cell renal cell carcinoma cells. J Biol Chem 295:15636-15649 (2020). PubMed: 32883810
    • Binch A  et al. Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues. JOR Spine 3:e1098 (2020). PubMed: 33015573
    • Souza SS  et al. Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF. PLoS One 15:e0241442 (2020). PubMed: 33147235
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Ki-67 staining in mouse prostate tissue using Abcam secondary antibody ab97049

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Ki-67 staining in mouse prostate tissue using Abcam secondary antibody Goat anti-Rabbit IgG H&L (Biotin) (ab97049)

    Abcam user community

    Verified customer

    Submitted Apr 08 2015

    Question

    Hi Abcam,

    Do you mind helping with this enquiry below?

    We have found another couple of secondary antibodies on your website that appear identical to AB6788 & AB6720 except for a different concentration.

    They are: ab97049 Goat anti-rabbit IgG H&L (biotin)

    and ab 97021 Goat polyclonal Secondary antibody to mouse IgG- H&L (Biotin)

    What we are hoping to do is to enhance a western blot signal by adding a streptavidin step prior to applying a biotinylated secondary antibody. (This has been successfully done by colleagues in the past).

    Would it be possible to get some advice as to which ones would be more suitable for our application?

    Thanks for your help :D

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 15 2012

    Answer

    Thank you for contacting us and you interest in our products.

    There are a few differences between the two pairs of antibodies you have identified. A few of these include the purification used (affitiny vs IgG fraction) as well as the ratio of conjugation. The ab6788 and ab6720 is estimated to have a conjugation ratio of 10-20 per IgG molecule whilst ab97049 and ab97021 have a ratio of 1:4.

    I am not familiar with the amplification technique you intend to use and cannot therefore anticipate how these differences may alter the results.

    If you could share a more detailed description of the procedure I may be able to advise in more detail.

    I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    Answered on Oct 15 2012

    Question

    Fluorescent detection with biotinylated secondary antibody is not giving strong signal. Would like to try avidin-biotin complex staining with DAB to amplify signal.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 08 2012

    Answer

    Thank you so much for your call today and for letting us know about the trouble with the staining.

    As we discussed, I'm sending a free of charge DAB avidin-biotin complex staining kit, ab64261, which should arrive on Monday on the order ***.

    Please keep me updated about the results after using the kit, and let me know if you have any questions or if there is anything else that we can do for you.

    Have a wonderful weekend!

    Read More

    Abcam Scientific Support

    Answered on Jun 08 2012

    Question

    J'ai commandé un anticorps anti cd31 ab28364 pour faire de l'immunohistochimie ainsi qu'un anticorps secondaire ab97049.

    J'aimerais savoir quel kit de révélation vous me conseiller pour révéler ce marquage en immuno histo

    et est ce que par la même occasion vous pouvez me faire parvenir un devis, pour les kits concernés.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 03 2012

    Answer

    Merci de nous avoir contactés.

    Les deux kits que je vous recommende sont :

    Streptavidin Alkaline Phosphatase (Ready to Use), www.abcam.com/ab64268
    Streptavidin Peroxidase (Ready to Use), www.abcam.com/ab64269



    Je vous fais parvenir un devis dans un autre email.

    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Read More

    Abcam Scientific Support

    Answered on Feb 03 2012

    Question

    HI

    I NEED CERTIFICATE OF ANALYSIS OF THE FOLLOWING PRODUCTS:

    CAT. NUMBER LOT

    AB27969 GR66929-1

    AB1793 GR66247-1

    AB2105 GR24376-3

    AB6672 GR58964-2

    AB34843 GR32935-5

    AB97049 GR46176-1

    THANKS

    Read More

    Abcam community

    Verified customer

    Asked on Feb 01 2012

    Answer

    Thank you for contacting Abcam.

    Please find attached the requested Certificate of Compliances.

    If there is anything else I can help you, please let me know.

    Read More

    Abcam Scientific Support

    Answered on Feb 01 2012

    Question

    dear colleagues
    hallo
    can i ask about the optimal dilution of these products.
    1-ab33923
    2-ab6668
    3-ab6822
    4-ab7481
    5-ab97049

    Read More

    Abcam community

    Verified customer

    Asked on Jan 31 2012

    Answer

    Thank you for contacting Abcam.

    As with most antibodies the dilution that works best for you depends on multiple varaibles and so you should do some titrations to find the best for you, but I placed a good starting point next to each.

    1-ab33923 - IHC-P: 1/50
    2-ab6668 - ICC/IF: 1/1000, WB: 1/2000, ELISA: 1/50000
    3-ab6822 - WB: 1/2000, ELISA: 1/200000, ICC/IF: 1/1000, IHC-P:1/100
    4-ab7481 -10%
    5-ab97049 - IHC-P: 1/500, ICC/IF: 1/200, ELISA: 1/20000, WB: 1/10000.

    Ifthere is anythingelse I can help you with, or ifyou would like a starting concentration for an application that I did not list about, then please let me know.

    Read More

    Abcam Scientific Support

    Answered on Jan 31 2012

    Question

    Dear Technical Team, Hope this e-mail finds you well! Kindly arrange to send us the certificate of compliance (CoC) for the below Abcam products as desired by one of our customers : Cat No. Description ab97021 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) ab97049 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) ab97023 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) ab60314 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) ab97080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed ab65623 ECL Western Blotting Substrate Kit - 50 Tests ab52476 Rabbit monoclonal [EP1829Y] to CaMKII ab11800 Goat polyclonal to DCXR ab7260 Rabbit polyclonal to GFAP - Astrocyte Marker ab104224 Mouse monoclonal [1B7] to FOX3 ab76260 Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9) ab64230 Mounting Medium ab82477 Rabbit polyclonal to Adenosine A1 Receptor ab3462 Rabbit polyclonal to alpha 1 Adrenergic Receptor

    Read More

    Abcam community

    Verified customer

    Asked on Dec 21 2011

    Answer

    I am beginning to gather the CoC's you have requested. When you have a chance, please provide the lot number from each catalog number so I can include this information on the documentation. Thanks for the additional information.

    Read More

    Abcam Scientific Support

    Answered on Dec 21 2011

    Question

    Dear Abcam:   How many  test for IHQ-P can the client realized with these antibodies? I understand that it depend of the dilution, but aprx.   AB27969 AB1793 AB2105 AB6672 AB34843 AB97049 AB97263   I have problems with dilutions, I would like learn about it. Do you have some examples o documents for it?   Thanks and B

    Read More

    Abcam community

    Verified customer

    Asked on Oct 10 2011

    Answer

    Thank you for your email! The best way to figure out dilutions and number of tests is to look at the datasheet. In most cases we will have a recommended dilution for each application, and will also include the concentration of the product along with the amount of product in the vial. Using these pieces of information, you can get the number of experiments that you will be able to do with that product. In order to give you the number of tests, I will need to know what application you are looking to use the antibodies in. The calculator at the following link may help you: https://www.msu.edu/~nixonjos/more/dilution.html?stock=1&needed=0.0001&volume=1&total=0.1 To get the number of tests, you can divide the final result ("total stock solution to use") by the amount included in the tube. I hope this helps. If you have any further questions, please do not hesitat

    Read More

    Abcam Scientific Support

    Answered on Oct 10 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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