Product nameGoat Anti-Rabbit IgG H&L (Chromeo™ 546)
See all IgG secondary antibodies
Specificityab60317 reacts with whole molecular rabbit IgG and the light chains of other rabbit immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins observed.
Tested applicationsSuitable for: ICC/IFmore details
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.013% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Conjugation notesChromeo™ 546 is spectrally similar to Cy3®. Conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching. Chromeo™ 546 conjugated antibodies have absorption and emission maxima of approximately 545 and 568 nm which are compatible with common excitation sources and filter sets. Molar extinction Coefficient: 96,800 M-1cm-1 (measured at Amax) Quantum Yield: ~ 13 % Excitation Wavelength Range: 530 to 550 nm Emission Wavelength Range: 560 to 570 nm
Our Abpromise guarantee covers the use of ab60317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Plate-based assays: 1/800 to 1/1000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This product has been referenced in:
- Zhang X et al. Human Gingiva-Derived Mesenchymal Stem Cells Modulate Monocytes/Macrophages and Alleviate Atherosclerosis. Front Immunol 9:878 (2018). Read more (PubMed: 29760701) »
- Valério-Gomes B et al. The Absolute Number of Oligodendrocytes in the Adult Mouse Brain. Front Neuroanat 12:90 (2018). Read more (PubMed: 30425626) »