Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)

Reviews (1)Q&A (1)References (28)
Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)
  • Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)
  • Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)

Key features and details

  • Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed
  • Conjugation: Cy2 ®. Ex: 489nm, Em: 505nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: WB, ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA

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Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details

  • Immunogen

    Full length native Rabbit IgG (purified).

  • Conjugation

    Cy2 ®. Ex: 489nm, Em: 505nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.

  • Conjugation notes

    Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 2.0 moles Cy2 per mole of Goat IgG

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.

    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent dilution.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC (1)
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.
Notes
WB
Use at an assay dependent dilution.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.

Images

  • Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)
    Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)
    Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution + Rabbit IgG at 0.05 µg


    Incubated with ab6940 for 1 hour at room temperature.

  • Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)
    Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)
    All lanes : Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution

    Lane 1 : Rabbit IgG whole molecule
    Lane 2 : Rabbit IgG F(ab) fragment
    Lane 3 : Rabbit IgG F(c) fragment
    Lane 4 : Rabbit IgM whole molecule
    Lane 5 : Normal rabbit serum

    Lysates/proteins at 0.05 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated donkey anti-goat IgG at 1/40000 dilution

    Performed under reducing conditions.

    Observed band size: 23,25,50,70 kDa why is the actual band size different from the predicted?



    Incubated with the primary antibody for 1 hour at room temperature.

    Incubated with the secondary antibody for 30 minutes at room temperature.

  • Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)
    Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)

    ab6940 was used at dilution 1/10000 with the primary antibody ab47840 in WB. See the review on ab47840.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (28)

Publishing research using ab6940? Please let us know so that we can cite the reference in this datasheet.

ab6940 has been referenced in 28 publications.

  • Ren Y  et al. miR-126-5p regulates H9c2 cell proliferation and apoptosis under hypoxic conditions by targeting IL-17A. Exp Ther Med 21:67 (2021). PubMed: 33365067
  • Fan B  et al. Mesenchymal stromal cell-derived exosomes ameliorate peripheral neuropathy in a mouse model of diabetes. Diabetologia 63:431-443 (2020). PubMed: 31740984
  • Li K  et al. MicroRNA-27a-3p promotes epithelial-mesenchymal transition by targeting NOVA alternative splicing regulator 1 in gastric cancer. Mol Med Rep 21:1615-1622 (2020). PubMed: 32016460
  • Wu L  et al. Dexmedetomidine protects PC12 cells from oxidative damage through regulation of miR-199a/HIF-1a. Artif Cells Nanomed Biotechnol 48:506-514 (2020). PubMed: 32024386
  • Liang X & Xu W miR-181a-5p regulates the proliferation and apoptosis of glomerular mesangial cells by targeting KLF6. Exp Ther Med 20:1121-1128 (2020). PubMed: 32742352
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Secondary Antibody for Immunocytochemisty with 2,2'-thiodiethanol (TDE) Mounting

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry
We have used this secondary antibody for immunocytochemistry with 2,2'-thiodiethanol (TDE) mounting media. TDE quenches the fluorescence of conventional green fluorophores (e.g., Alexafluor 488, GFP, etc....), but the original description of this mounting media (PMID: 17131355) reported an increase in the relative quantum yield of Cy3. We tried this Cy2-conjugated secondary antibody following TDE mounting of stained HEK293 cells and saw excellent signal from the green channel. We did not observe bleaching of the signal following Z-stack image acquisition.

The protocol for obtaining the image shown in the figure was a standard ICC protocol, followed by the TDE mounting procedure from the original TDE paper (PMID: 17131355), using sequential immersions in increasing concentrations of TDE. Slides were imaged on a Zeiss Observer Z1 with LED-based excitation (488 nm). Acquired images were deconvolved and the included image is a maximum intensity projection.

Dr. Sam Nowitzki

Verified customer

Submitted Jul 09 2015

Question

I am looking for a secondary antibody anti-Rabbit to used with Anti-GFAP antibody - Astrocyte Marker cat. no. ab7779. I would like to know if use Goat anti-Rabbit IgG H&L (Cy2 ®) secondary antibody cat. no.ab6940 or Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed cat. no. ab97075 it will work and what does "pre-adsorbed" mean ? Thank you

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Answer

Thank you for contacting us.

Yes, either ab6940 or ab97075 will work as a suitable secondary with ab7779. "Pre-adsorbed" means that we pre-treated the secondary antibody with normal sera from certain species to remove cross-reactivity with those species. In the example of ab97075, due to this process, this secondary will show minimal cross-reactivity with IgG from Chicken, Cow, Horse, Human, Mouse, Pig, and Rat. This comes in handy if your sample is from one of these species and you don't want the secondary binding to the endogenous IgG present in the sample.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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