Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)
Key features and details
- Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed
- Conjugation: Cy2 ®. Ex: 489nm, Em: 505nm
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA
Related conjugates and formulations
Overview
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Product name
Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
Immunogen
Full length native Rabbit IgG (purified).
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Conjugation
Cy2 ®. Ex: 489nm, Em: 505nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. -
Conjugation notes
Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 2.0 moles Cy2 per mole of Goat IgG -
Clonality
Polyclonal -
Isotype
IgG -
General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent dilution.
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| ICC/IF |
1/1000 - 1/5000.
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| IHC-P |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| ICC | (1) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent dilution.
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| ELISA |
1/10000 - 1/50000.
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| Notes |
|---|
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WB
Use at an assay dependent dilution. |
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ICC/IF
1/1000 - 1/5000. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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ICC
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent dilution. |
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ELISA
1/10000 - 1/50000. |
Images
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Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution + Rabbit IgG at 0.05 µg
Incubated with ab6940 for 1 hour at room temperature.
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Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)All lanes : Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution
Lane 1 : Rabbit IgG whole molecule
Lane 2 : Rabbit IgG F(ab) fragment
Lane 3 : Rabbit IgG F(c) fragment
Lane 4 : Rabbit IgM whole molecule
Lane 5 : Normal rabbit serum
Lysates/proteins at 0.05 µg per lane.
Secondary
All lanes : Peroxidase-conjugated donkey anti-goat IgG at 1/40000 dilution
Performed under reducing conditions.
Observed band size: 23,25,50,70 kDa why is the actual band size different from the predicted?Incubated with the primary antibody for 1 hour at room temperature.
Incubated with the secondary antibody for 30 minutes at room temperature.
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Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (29)
ab6940 has been referenced in 29 publications.
- Ren Y et al. miR-126-5p regulates H9c2 cell proliferation and apoptosis under hypoxic conditions by targeting IL-17A. Exp Ther Med 21:67 (2021). PubMed: 33365067
- Liu Y et al. STAT4-mediated down-regulation of miR-3619-5p facilitates stomach adenocarcinoma by modulating TBC1D10B. Cancer Biol Ther 21:656-664 (2020). PubMed: 32397798
- Fan B et al. Mesenchymal stromal cell-derived exosomes ameliorate peripheral neuropathy in a mouse model of diabetes. Diabetologia 63:431-443 (2020). PubMed: 31740984
- Li K et al. MicroRNA-27a-3p promotes epithelial-mesenchymal transition by targeting NOVA alternative splicing regulator 1 in gastric cancer. Mol Med Rep 21:1615-1622 (2020). PubMed: 32016460
- Wu L et al. Dexmedetomidine protects PC12 cells from oxidative damage through regulation of miR-199a/HIF-1a. Artif Cells Nanomed Biotechnol 48:506-514 (2020). PubMed: 32024386