Product nameGoat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed
See all IgG secondary antibodies
DescriptionGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy2 ®), pre-adsorbed
Tested applicationsSuitable for: WB, ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Full length native Rabbit IgG (purified).
ConjugationCy2 ®. Ex: 489nm, Em: 505nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Conjugation notesCy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 2.0 moles Cy2 per mole of Goat IgG
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
Our Abpromise guarantee covers the use of ab6940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
|ICC/IF||1/1000 - 1/5000.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent dilution.|
|ELISA||1/10000 - 1/50000.|
Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution + Rabbit IgG at 0.05 µg
Incubated with ab6940 for 1 hour at room temperature.
All lanes : Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution
Lane 1 : Rabbit IgG whole molecule
Lane 2 : Rabbit IgG F(ab) fragment
Lane 3 : Rabbit IgG F(c) fragment
Lane 4 : Rabbit IgM whole molecule
Lane 5 : Normal rabbit serum
Lysates/proteins at 0.05 µg per lane.
All lanes : Peroxidase-conjugated donkey anti-goat IgG at 1/40000 dilution
Performed under reducing conditions.
Observed band size: 23,25,50,70 kDa why is the actual band size different from the predicted?
Incubated with the primary antibody for 1 hour at room temperature.
Incubated with the secondary antibody for 30 minutes at room temperature.
This product has been referenced in:
- Wang G et al. Downregulation of microRNA-183-5p inhibits the proliferation and invasion of colorectal cancer cells by inactivating the reticulocalbin-2/Wnt/ß-catenin signaling pathway. Mol Med Rep 19:4475-4483 (2019). Read more (PubMed: 30896885) »
- Tan P et al. Rapamycin-induced miR-30a downregulation inhibits senescence of VSMCs by targeting Beclin1. Int J Mol Med 43:1311-1320 (2019). Read more (PubMed: 30747228) »