Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Full length native Rabbit IgG (purified).

  • Conjugation
    Cy3 ®. Ex: 552nm, Em: 565nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
  • Conjugation notes
    Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 9.9 moles Cy3 per mole of Goat IgG
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labelled without unwanted cross reaction.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-Fr Use at an assay dependent dilution.
IHC-P 1/100. PubMed: 17045908
ICC Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.

Images

  • Histone modifications associated with NOR-1 and NOR-2 loci in root tip cycling cells of Q.robur.

    IFF detection of H3K9ac (A), H3K4me3 (B), H3K9me1 (C) and H3K27me2 (D). Green signals correspond to immunofluorescence of histone antibodies and red signals represent 18S rDNA FISH signals. Letter “n” marks the nucleoli. Scale bar is 5 µm.

  • Photomicrographs showing immunostaining of (Panel A) ABCG2 (red), (Panel B) Bmi-1 (green), (Panel C) C/EBPδ (red), (Panel D) PCNA (red), (Panel E) CK18 (green), and (Panel F) cleaved caspase-3 (red) in HOK (human oral keratinocytes) cells cultured for five days without subsequent storage (control).

    Photomicrographs are representative of four independent samples.

    Cell nuclei were counterstained with DAPI (blue).

    Original magnification: 200x.

  • Examples of the different antibody stainings for functional analyses.

    Left Panel: Ki-67 staining (Cy-3, red), HEK-293 transfected with NKX2-5-YFP (green).

    Right Panel: Cyclin B staining (Cy-3, red), PANC-1 transfected with PRKCZ-YFP (green).

    Original magnifications are indicated in the images.

  • ab6939 was used at dilution 1/500 with the primary antibody ab10812 in ICC/IF. See the review on ab10812.

  • Lane M: 3 µL Molecular ladder.

    Lane 1: Rabbit IgG whole molecule (p/n 011-0102).

    Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105).

    Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103).

    Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107).

    Lane 5: Normal Rabbit Serum (p/n B309).

    All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.

    Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt and Sh Serum Proteins) used at 1:1,000 for 60 min at RT.

    Secondary antibody: ab6939 used at 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.

    Rabbit F(c) migrates slightly higher.

  • Lane 1: Rabbit IgG.

    Load: 50 ng per lane.

    Secondary antibody: ab6939 at 1:1,000 for 60 min at RT. Blocked for 30 min at RT.

    Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Rabbit IgG.

References

This product has been referenced in:
  • Gao J  et al. In vitro investigation of the mechanism underlying the effect of ginsenoside on the proliferation and differentiation of neural stem cells subjected to oxygen-glucose deprivation/reperfusion. Int J Mol Med 41:353-363 (2018). Read more (PubMed: 29138802) »
  • Mladenova D  et al. Adar3 Is Involved in Learning and Memory in Mice. Front Neurosci 12:243 (2018). Read more (PubMed: 29719497) »
See all 53 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your inquiry.

We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

Please find a list of the product code and the links to the respective datasheets below:

ab1012 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=1012 (or use the following: https://www.abcam.com/index.html?datasheet=1012).

ab12209 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=12209 (or use the following: https://www.abcam.com/index.html?datasheet=12209).

ab6002 (Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=6002 (or use the following: https://www.abcam.com/index.html?datasheet=6002).

ab124462 (Anti-Flag Tag antibody)

https://www.abcam.com/index.html?datasheet=124462 (or use the following: https://www.abcam.com/index.html?datasheet=124462).

ab6939 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=6939 (or use the following: https://www.abcam.com/index.html?datasheet=6939).

ab96899 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=96899 (or use the following: https://www.abcam.com/index.html?datasheet=96899).

ab96919 (Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/index.html?datasheet=96919 (or use the following: https://www.abcam.com/index.html?datasheet=96919).

ab96879 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=96879 (or use the following: https://www.abcam.com/index.html?datasheet=96879).

ab96871 (Goat anti-Mouse IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/index.html?datasheet=96871 (or use the following: https://www.abcam.com/index.html?datasheet=96871).

ab97265 (Goat anti-Mouse IgG Fc (HRP) secondary antibody)

https://www.abcam.com/index.html?datasheet=97265 (or use the following: https://www.abcam.com/index.html?datasheet=97265).

ab7481 (Goat Serum)

https://www.abcam.com/index.html?datasheet=7481 (or use the following: https://www.abcam.com/index.html?datasheet=7481).

ab7487 (Rabbit Serum (sterile))

https://www.abcam.com/index.html?datasheet=7487 (or use the following: https://www.abcam.com/index.html?datasheet=7487).

ab46666 (Herring sperm ssDNA)

https://www.abcam.com/index.html?datasheet=46666 (or use the following: https://www.abcam.com/index.html?datasheet=46666).

ab52579 (Ribonuclease A protein (Active))

https://www.abcam.com/index.html?datasheet=52579 (or use the following: https://www.abcam.com/index.html?datasheet=52579).

ab73430 (DNase I protein (Active))

https://www.abcam.com/index.html?datasheet=73430 (or use the following: https://www.abcam.com/index.html?datasheet=73430).

ab64220 (Proteinase K)

https://www.abcam.com/index.html?datasheet=64220 (or use the following: https://www.abcam.com/index.html?datasheet=64220).

ab65621 (Protease Inhibitor Cocktail)

https://www.abcam.com/index.html?datasheet=65621 (or use the following: https://www.abcam.com/index.html?datasheet=65621).

ab116028 (Prism Ultra Protein Ladder (10-245 kDa))

https://www.abcam.com/index.html?datasheet=116028 (or use the following: https://www.abcam.com/index.html?datasheet=116028).


I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.

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Question
Answer

Thank you very much for the reply.

It is clear from the images provided that the customer has observed some background with the secondary antibody. In order to reduce this, I would advise reducing the concentration which is currently being used. We would recommend initially trying 1/1000-1/5000 for the experiment the customer is currently trying.

I hope this information has been of help. If the customer continues to have problems following this advice please do let us know and we will endeavor to help further.

I hope you ave a nice weekend.

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Question
Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In order to understand what may be contributing to the problems you have encountered, could you please provide me with a few additional details:

1. Which primary antibody was used in the staining? Catalogue number and supplier?

2. Which other secondary antibody was used? Catalogue number and supplier?

3. Would it be possible to provide some images of the staining produced with the ab6939 and the alternative antibody you used in order to understand the differences observed?

4. Could you please provide me with the order number on which ab6939 was purchased?

I look forward to receiving your reply.

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