Product nameGoat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
See all IgG secondary antibodies
Tested applicationsSuitable for: ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISAmore details
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Full length native Rabbit IgG (purified).
ConjugationCy3 ®. Ex: 552nm, Em: 565nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Conjugation notesCy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 9.9 moles Cy3 per mole of Goat IgG
General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labelled without unwanted cross reaction.
Our Abpromise guarantee covers the use of ab6939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/5000.|
|IHC-Fr||Use at an assay dependent dilution.|
|IHC-P||1/100. PubMed: 17045908|
|ICC||Use at an assay dependent dilution.|
|Flow Cyt||1/500 - 1/2500.|
|ELISA||1/10000 - 1/50000.|
Histone modifications associated with NOR-1 and NOR-2 loci in root tip cycling cells of Q.robur.
IFF detection of H3K9ac (A), H3K4me3 (B), H3K9me1 (C) and H3K27me2 (D). Green signals correspond to immunofluorescence of histone antibodies and red signals represent 18S rDNA FISH signals. Letter “n” marks the nucleoli. Scale bar is 5 µm.
Photomicrographs showing immunostaining of (Panel A) ABCG2 (red), (Panel B) Bmi-1 (green), (Panel C) C/EBPδ (red), (Panel D) PCNA (red), (Panel E) CK18 (green), and (Panel F) cleaved caspase-3 (red) in HOK (human oral keratinocytes) cells cultured for five days without subsequent storage (control).
Photomicrographs are representative of four independent samples.
Cell nuclei were counterstained with DAPI (blue).
Original magnification: 200x.
Examples of the different antibody stainings for functional analyses.
Left Panel: Ki-67 staining (Cy-3, red), HEK-293 transfected with NKX2-5-YFP (green).
Right Panel: Cyclin B staining (Cy-3, red), PANC-1 transfected with PRKCZ-YFP (green).
Original magnifications are indicated in the images.
Lane M: 3 µL Molecular ladder.
Lane 1: Rabbit IgG whole molecule (p/n 011-0102).
Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105).
Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103).
Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107).
Lane 5: Normal Rabbit Serum (p/n B309).
All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.
Primary Antibody: ab6939 used at 1/1000 for 60 min at RT.
Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody used at 1/40000 in blocking buffer for 30 min at RT.
Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.
Rabbit F(c) migrates slightly higher.
Lane 1: Rabbit IgG.
Load: 50 ng per lane.
Secondary antibody: ab6939 at 1/1000 for 60 min at RT. Blocked for 30 min at RT.
Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Rabbit IgG.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6939 has been referenced in 83 publications.
- Hu X et al. Silencing of Long Non-coding RNA HOTTIP Reduces Inflammation in Rheumatoid Arthritis by Demethylation of SFRP1. Mol Ther Nucleic Acids 19:468-481 (2020). PubMed: 31902746
- Li Y et al. mPGES-1/PGE2 promotes the growth of T-ALL cells in vitro and in vivo by regulating the expression of MTDH via the EP3/cAMP/PKA/CREB pathway. Cell Death Dis 11:221 (2020). PubMed: 32251289
- Zhang HY et al. Early growth response 1 reduction in peripheral blood involving condylar subchondral bone loss. Oral Dis 25:1759-1768 (2019). PubMed: 31357246
- Wang G et al. Minocycline Preserves the Integrity and Permeability of BBB by Altering the Activity of DKK1-Wnt Signaling in ICH Model. Neuroscience 415:135-146 (2019). PubMed: 31344398
- Zhang S et al. Profiling the long noncoding RNA interaction network in the regulatory elements of target genes by chromatin in situ reverse transcription sequencing. Genome Res 29:1521-1532 (2019). PubMed: 31315906