Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
    See all IgG secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed
  • Host species

    Goat
  • Target species

    Rabbit
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.

     

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Horse, Human, Mouse, Pig, Rat more details
  • Immunogen

    Other Immunogen Type corresponding to Rabbit IgG.

  • Conjugation

    Cy3 ®. Ex: 552nm, Em: 565nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3 ®.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/100 - 1/1000.

Images

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

     

    Preparation:
    Fix in 3%PFA in PBS for 30 min at RT
    Incubate in 7.5% sucrose-PBS for 3h at RT
    Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    Embed the EBs in tissue-Tek OCT compound
    Cut frozen sections to 4-20 µm thickness

     

    Primary antibody: Rabbit anti-laminin alpha 1, 1:400

     

    Secondary antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3®) (ab97075), 1:100
    Nuclei were counterstained with SYTO 16

     

     

  • Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

    Sample: mouse endoderm cell

    Preparation:

    Fix in 3% PFA in PBS for 30 min at RT

    Primary antibody: Rabbit anti-talin (ab71333), 1:100

    Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3® 488) preadsorbed (ab97075), 1:100

References

This product has been referenced in:

  • Resende TP  et al. Evaluation of mouse enteroids as a model for Lawsonia intracellularis infection. Vet Res 50:57 (2019). Read more (PubMed: 31324204) »
  • Ogata S  et al. Changes of Blood-Brain Barrier and Brain Parenchymal Protein Expression Levels of Mice under Different Insulin-Resistance Conditions Induced by High-Fat Diet. Pharm Res 36:141 (2019). Read more (PubMed: 31367840) »
See all 10 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.

1. The alternative to Alexa Fluor® 488 goat anti-mouse Ig Cat # G A-11001 is ab96871; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-ab96871.html

or ab96879; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-pre-adsorbed-ab96879.html

2. ab96895; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-pre-adsorbed-ab96895.html

3. ab97035; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Cy3-pre-adsorbed-ab97035.html

4. ab97075; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-Cy3-pre-adsorbed-ab97075.html

5. ab10345; https://www.abcam.com/PSTAIR-antibody-PSTAIR-ab10345.html

6. We do not have anti Nanog mouse monoclonal that customer is after. We have similar rabbit polyclonal antibodies available. The links are

https://www.abcam.com/Nanog-antibody-ab70482.html

https://www.abcam.com/Nanog-antibody-ab90640.html

7. ab109187; https://www.abcam.com/Oct4-antibody-EPR2053-2-ab109187.html

8. ab97959; https://www.abcam.com/SOX2-antibody-ab97959.html

9. ab131000; https://www.abcam.com/Caspase-3-antibody-ab131000.html

10. ab18737; https://www.abcam.com/Caspase-2-antibody-ab18737.html

11. We do not sell this chemical.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting Abcam and for your interest in our products.


All three of the secondary antibodies you have suggested are compatible with the primary antibody ab7779. In terms of the color of the staining, you can expect the following:



ab97075 (Cy3) - Yellow

ab6940 (Cy2) - Green

ab97077 (Cy5) - Red



Did you have a specific color in mind for experiments? The choice of color will also depend largely on the filters available for your microscope. Please be sure to check the filters against the absorbance and emission spectra for these products: https://www.abcam.com/index.html?pageconfig=resource&rid=11444



I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

Read More

Answer

Thank you for contacting us.

Yes, either ab6940 or ab97075 will work as a suitable secondary with ab7779. "Pre-adsorbed" means that we pre-treated the secondary antibody with normal sera from certain species to remove cross-reactivity with those species. In the example of ab97075, due to this process, this secondary will show minimal cross-reactivity with IgG from Chicken, Cow, Horse, Human, Mouse, Pig, and Rat. This comes in handy if your sample is from one of these species and you don't want the secondary binding to the endogenous IgG present in the sample.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up