Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed
    See all IgG secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3.5 ®), pre-adsorbed
  • Host species

    Goat
  • Target species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Full length native Rabbit IgG (purified).

  • Conjugation

    Cy3.5 ®. Ex: 581nm, Em: 596nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above
  • Conjugation notes

    Cy3.5 (Cyanine 3.5-OSu) (Molecular Weight 1286 daltons) Absorption Wavelength: 581 nm Emission Wavelength: 596 nm Fluorochrome/Protein Ratio: 9.3 moles Cy3.5 per mole of Goat IgG
  • Clonality

    Polyclonal
  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

References

This product has been referenced in:

  • Weingarten G  et al. Insulin receptor plays a central role in skin carcinogenesis by regulating cytoskeleton assembly. FASEB J 33:2241-2251 (2019). Read more (PubMed: 30332298) »
  • Ramalho AF  et al. Dietary fats promote functional and structural changes in the median eminence blood/spinal fluid interface-the protective role for BDNF. J Neuroinflammation 15:10 (2018). IHC-P ; Mouse . Read more (PubMed: 29316939) »
See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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