Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (DyLight® 488)
    See all IgG secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488)
  • Host species

    Goat
  • Target species

    Rabbit
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Immunogen

    Other Immunogen Type corresponding to Rabbit IgG.

  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:

  • Zhu M  et al. Anti-inflammatory effect of stromal vascular fraction cells in fat transplantation. Exp Ther Med 17:1435-1439 (2019). Read more (PubMed: 30680025) »
  • Decourtye L  et al. Impact of insulin on primary arcuate neurons culture is dependent on early-postnatal nutritional status and neuronal subpopulation. PLoS One 13:e0193196 (2018). Read more (PubMed: 29466413) »
See all 12 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

It has been a pleasure to help. We are here until 8:00pm eastern time so not quite as late as the university crowd. Please feel free to contact me if you have any questions.

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ab18956 staining GDNF in rat brain tissue sections by IHC-P (Formalin/PFA-fixed paraffin-embedded sections). Cells were fixed with paraformaledhyde, permeabilized with 0.1% Tween-20 and PBS and blocked with 1% BSA for 30 minutes at 25°C antigen retrieval was by heat mediation in a citrate buffer pH 6.0. Samples were incubated with primary antibody (1/150 in TBS) for 24 hours at 4°C. ab96883 at a dilution of 1/200 was used as the secondary antibody.
( Green = GDNF, Blue = DAPI stained nuclei.)

Abcam user community

Verified customer

Submitted Dec 03 2015

Application
Immunomicroscopy
Goat anti-Rabbit IgG H&L (DyLight® 488) is good to use.

Abcam user community

Verified customer

Submitted Aug 07 2015

Answer

Thank you for your enquiry.

Could you confirm that Ki67 primary antibody you are referring to has product code ab16667?

I can confirm that for ab16667, ab125938 would be a suitable isotype control, and ab96883 would be a suitable secondary.

I can suggest to review the online datasheets to ensure these antibodies are tested in the application you are using. This will ensure they are covered by our 6 month guarantee.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for your enquiry.



Here are the answers to your quesitons.



1) "Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 547-577 of Human Albumin." means that the immunogen (amino acid range) used was taken from 547-577 of Human Albumin. So the antibody is specific to part of the human albumin chain.



2) I am not sure what you mean by passivization. Do you mean blocking solution? If so, you can also use the serum from the secondary antibody host species. In this case, if you are going to use ab96883, then please use 10% goat serum for blocking.



3) You did not mention what application you will be using the products in, so if it is a fluorescence related application like ICC/IF or Flow cytometry then yes, you can use this "Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488) (ab96883)" with ab135575.



If there is anything else that I can help you with please do not hesitate to contact me.


Have a nice day!

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Question
Answer

Thank you for calling earlier today.

Below is a list to some secondary antibodies that may be useful to you:

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-594-pre-adsorbed-ab96881.html
https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Cy3-pre-adsorbed-ab97035.html
https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Cy5-pre-adsorbed-ab97037.html
https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-550-pre-adsorbed-ab98786.html



If there is anything else I can help you with, please let me know.

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Question
Answer

Thank you for contacting Abcam. We have a large number of secondary antibodies with fluorescent tags. Theeasiest way to find the one that best fits your needs is from the Abcam home page. The secondary antibodies drop down, located in the yellow banner, has a listing of our DyLight conjugated secondary antibodies. You may then choose the conjugation that is best for your needs. In order to assist your search, I have chosen two products that may work for you. Both are against Rabbit IgG and will work in flow cytometry and IHC. These particular antibodies are conjugated to our DyLight fluorochromes but other fluorochromes are available. The product that I have chosen are ab96891 :https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-ab96891.html and ab96883: https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-ab96883.html In regards to your second question. Isotype controls are essential in flow cytometry and recommended in IHC.The appropriate isotype control for this product isab27478. This product has been tested in flow cytometry and IHC-P. I have included the link to a pdf which details our recommended controls for flow cytometry. I hope this information is useful. https://www.abcam.com/ps/pdf/protocols/flow_cytometry_controls.pdf

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Answer

Thank you for your email and interest in Abcam products. We currently do not have AlexaFluor conjugated antibodies or proteins available in our catalog. Having said this though, we have a number of products available that are conjugated to DyLight® fluorochromes. This family of dyes is comparable to AlexaFluor dyes regarding high fluorescence intensity, photostability and water solubility. Both the absorption and emission properties of the DyLight® fluorochomes are compatible with the excitation and detection wavelengths of common fluorescence instrumentation (https://www.abcam.com/DyLight). Please be reassured that all our products are covered by our Abpromise: We guarantee our products work in the tested species and applications as stated on the datasheet, and we will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase. Thus, please see a selection of our options: 1+2) Unfortunately, we currently do not have any phalloidin protein or antibody against phalloidin available. 3) ab96873, ab96881: Click here (or use the following: https://www.abcam.com/index.html?datasheet=96873). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96881). 4) ab96871, ab96871, ab97022: Click here (or use the following: https://www.abcam.com/index.html?datasheet=96871). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96871). Click here (or use the following: https://www.abcam.com/index.html?datasheet=97022). 5) ab102883, ab102884, ab102885 (please see https://www.abcam.com/EasyLink or attachment for more details): Click here (or use the following: https://www.abcam.com/index.html?datasheet=102883). Click here (or use the following: https://www.abcam.com/index.html?datasheet=102884). Click here (or use the following: https://www.abcam.com/index.html?datasheet=102885). 6) ab102923, ab1029234, ab102925 (please see https://www.abcam.com/EasyLink or attachment for more details): Click here (or use the following: https://www.abcam.com/index.html?datasheet=102923). Click here (or use the following: https://www.abcam.com/index.html?datasheet=102924). Click here (or use the following: https://www.abcam.com/index.html?datasheet=102925). 7) ab97050, ab96883, ab96899: Click here (or use the following: https://www.abcam.com/index.html?datasheet=97050). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96883). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96899). 8) ab96885, ab96897: Click here (or use the following: https://www.abcam.com/index.html?datasheet=96885). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96897). We also have the secondary antibodies in different formats (pre-absorbed, Fab fragments etc) which might be more suitable for certain applications. I will be happy to assist you in finding the most appropriate product on request. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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