Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, IHC-Fr, WBmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Horse, Human, Mouse, Pig, Rat more details
  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab202372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-Fr 1/200.

following abreview ab96895

WB 1/1000 - 1/20000.

5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended.

Images

  • Immunofluorescent analysis of Human stomach carcinoma labeling PCNA with purified rabbit anti-PCNA antibody at 1/100 dilution followed by ab202372 secondary antibody at 1/100 dilution (green). Counterstain: Dapi (Blue). Images represent different lots of ab202372.

  • All lanes : cocktail of rabbit anti-Actin and mouse anti-hnRNP at 1 µg/ml

    Lane 1 : HeLa cell lysate at 30 µg
    Lane 2 : HeLa cell lysate at 15 µg

    Secondary
    All lanes : cocktail of Dylight® 488-conjugated goat anti-rabbit (ab202372) (blue) and Dylight® 680-conjugated goat anti-mouse (red) at 0.5 µg/ml

References

This product has been referenced in:

  • Pilia M  et al. Influence of substrate curvature on osteoblast orientation and extracellular matrix deposition. J Biol Eng 7:23 (2013). ICC/IF . Read more (PubMed: 24090183) »
  • Xu Y  et al. Pseudomonas aeruginosa pyocyanin activates NRF2-ARE-mediated transcriptional response via the ROS-EGFR-PI3K-AKT/MEK-ERK MAP kinase signaling in pulmonary epithelial cells. PLoS One 8:e72528 (2013). ICC/IF . Read more (PubMed: 24015256) »
See all 2 Publications for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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