Product nameGoat Anti-Rabbit IgG H&L (DyLight® 550)
See all IgG secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Tested applicationsSuitable for: ICC/IF, IHC-P, Flow Cytmore details
Other Immunogen Type corresponding to Rabbit IgG.
ConjugationDyLight® 550. Ex: 562nm, Em: 576nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
General notesDyLight® 550 replaces DyLight® 549.
Our Abpromise guarantee covers the use of ab96884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/500.|
|IHC-P||1/50 - 1/500.
DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.
|Flow Cyt||1/50 - 1/200.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab96884 has been referenced in 10 publications.
- Galoian K et al. Toll like receptors TLR1/2, TLR6 and MUC5B as binding interaction partners with cytostatic proline rich polypeptide 1 in human chondrosarcoma. Int J Oncol 52:139-154 (2018). PubMed: 29138803
- Hart CR et al. Increased skeletal muscle mitochondrial free radical production in peripheral arterial disease despite preserved mitochondrial respiratory capacity. Exp Physiol 103:838-850 (2018). PubMed: 29604234
- Liu L et al. Neurexin Restricts Axonal Branching in Columns by Promoting Ephrin Clustering. Dev Cell 41:94-106.e4 (2017). PubMed: 28366281
- Takeuchi Y et al. Development of Novel Mouse Model of Ulcers Induced by Implantation of Magnets. Sci Rep 7:4843 (2017). IHC-P . PubMed: 28687753
- Cen M et al. TIEG1 deficiency confers enhanced myocardial protection in the infarcted heart by mediating the Pten/Akt signalling pathway. Int J Mol Med 39:569-578 (2017). PubMed: 28204828
- Arora K et al. Behavioral alterations are associated with vitamin B12 deficiency in the transcobalamin receptor/CD320 KO mouse. PLoS One 12:e0177156 (2017). PubMed: 28545069
- Ueno K et al. Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblasts. Sci Rep 6:28538 (2016). IHC-P . PubMed: 27329845
- Nakamura T et al. Influence of aging on the quantity and quality of human cardiac stem cells. Sci Rep 6:22781 (2016). IHC ; Human . PubMed: 26947751
- Faulkner-Jones A et al. Bioprinting of human pluripotent stem cells and their directed differentiation into hepatocyte-like cells for the generation of mini-livers in 3D. Biofabrication 7:044102 (2015). PubMed: 26486521
- Gifford JR et al. Quadriceps exercise intolerance in patients with chronic obstructive pulmonary disease: the potential role of altered skeletal muscle mitochondrial respiration. J Appl Physiol (1985) 119:882-8 (2015). PubMed: 26272320