Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96902)

Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed
    See all IgG secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 650), pre-adsorbed
  • Host species

    Goat
  • Target species

    Rabbit
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.

     

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details
  • Conjugation

    DyLight® 650. Ex: 654nm, Em: 673nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    DyLight® 650 replaces DyLight® 649.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.

DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.

ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:

  • Hirukawa M  et al. Development of a Tissue-Engineered Artificial Ligament: Reconstruction of Injured Rabbit Medial Collateral Ligament With Elastin-Collagen and Ligament Cell Composite Artificial Ligament. Artif Organs 42:736-745 (2018). Read more (PubMed: 29660790) »
  • Cox D & Ecroyd H The small heat shock proteins aB-crystallin (HSPB5) and Hsp27 (HSPB1) inhibit the intracellular aggregation of a-synuclein. Cell Stress Chaperones 22:589-600 (2017). Read more (PubMed: 28337642) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

As discussed over the phone I have looked at the protocols we have for double staining to see if I can suggest anything further to our conversation. As discussed the protocol that we have on our website is relatively simple, with sequential incubation of the primary antibodies with individual blocking and washing steps between each incubation. https://www.abcam.com/index.html?pageconfig=resource&rid=11458 A protocol from IHC world recommends much the same: http://www.ihcworld.com/_protocols/general_IHC/immunofl_double_squential.htm As discussed I would initially optimise each of your staining individually and once you are happy with them combine the two protocols. It may be that you experience some non-specific staining due to the two primary antibodies being from the same species. This may be optimised with the blocking steps used. Alternatively the use of unconjugated Fab antibody fragments may be employed, either to "change the species" of one of the primary antibodies or to block the free sites of the secondary antibody first applied. If you would like more detail on how to apply this I can send you the protocols where this is described more fully. As for the staining you are currently performing with ab75850, if you do not see an improvement with the suggestions discussed with the positive control tissue please do let me know.

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