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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rabbit IgG H&L (FITC) (ab6717)

  • Datasheet
  • SDS
Reviews (2)Q&A (21)References (253)

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (FITC) (ab6717)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (FITC) (ab6717)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (FITC) (ab6717)
  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (FITC) (ab6717)

Key features and details

  • Goat Anti-Rabbit IgG H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-FoFr, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA

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Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rabbit
  • Tested applications

    Suitable for: IHC-FoFr, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details
  • Immunogen

    Rabbit IgG whole molecule

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Fluorophore
    • FITC

Associated products

  • Related Products

    • Normal Goat Serum (ab7481)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
Use at an assay dependent concentration. PubMed: 24216136
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
1/500 - 1/2500.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF (1)
1/1000 - 1/5000.
ELISA
1/10000 - 1/50000.
Notes
IHC-FoFr
Use at an assay dependent concentration. PubMed: 24216136
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
1/500 - 1/2500.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
ELISA
1/10000 - 1/50000.

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (FITC) (ab6717)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (FITC) (ab6717)Kumar S et al. The role of tumour suppressor PDCD4 in beta cell death in hypoxia. PLoS One 12:e0181235 (2017). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Subcellular localisation of PDCD4 was assessed by immunocytochemical staining. ARIP cells were seeded at 3×105 cells/well on sterile cover slips in six well plates and incubated overnight under standard culture conditions.

    Following specific experimental conditions, media was removed and cells were washed with PBS and fixed with 3.7% formalin. Cells were then permeabilised with 0.1% triton X-100 in PBS followed by blocking in blocking buffer (10% Goat serum; 2% BSA; 0.2% Tween 20; 0.7% Glycerol in PBS) for 1 hour. Cells were incubated overnight with ab51495 at 1/100 dilution followed by ab6717 (green) at 1/80 dilution. The nuclear counterstain is DAPI (Blue).

    Results are representative of three separate experiments and images were representative of six separate fields. PDCD4 localized and expressing At G0: Cytoplasmic and very low expression; At N12: Cytoplasmic and low expression; At N24: very high cytoplasmic and low nuclear expression; At H12: Cytoplasmic expression and At H24 very high cytoplasmic and low nuclear expression. Over-all PDCD4 was highly expressed in the cytoplasm, with very low expression under serum starved conditions

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (FITC) (ab6717)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (FITC) (ab6717)This image is courtesy of an anonymous Abreview
    ab11320 (Rabbit polyclonal to gamma Tubulin - Centrosome Marker) at 1/400 dilution staining human neuroblastoma cells by ICC/IF. The cells were fixed with paraformaldehyde and blocked with serum and then incubated with the primary antibody for 16 hours. ab6717 was used as the secondary.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (FITC) (ab6717)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (FITC) (ab6717)
    Ab6717 was used at dilution 1/200 with the primary antibody ab36823 in IHC-P. See the review on ab36823.
  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (FITC) (ab6717)
    Flow Cytometry - Goat Anti-Rabbit IgG H&L (FITC) (ab6717)
    Ab6717 was used with the primary antibody ab66506 in Flow Cyt. See Abreview on ab66506.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (253)

Publishing research using ab6717? Please let us know so that we can cite the reference in this datasheet.

ab6717 has been referenced in 253 publications.

  • Wang X  et al. Hypoxic mast cells accelerate the proliferation, collagen accumulation and phenotypic alteration of human lung fibroblasts. Int J Mol Med 45:175-185 (2020). PubMed: 31746371
  • Xu G  et al. Up-regulated microRNA-33b inhibits epithelial-mesenchymal transition in gallbladder cancer through down-regulating CROCC. Biosci Rep 40:N/A (2020). PubMed: 31799620
  • Saribas AS  et al. A comprehensive proteomics analysis of JC virus Agnoprotein-interacting proteins: Agnoprotein primarily targets the host proteins with coiled-coil motifs. Virology 540:104-118 (2020). PubMed: 31765920
  • Zheng L  et al. Inhibition of TIM-4 protects against cerebral ischaemia-reperfusion injury. J Cell Mol Med 24:1276-1285 (2020). PubMed: 31774937
  • Lv Z  et al. Integrin a7 correlates with worse clinical features and prognosis, and its knockdown inhibits cell proliferation and stemness in tongue squamous cell carcinoma. Int J Oncol 56:69-84 (2020). PubMed: 31789398
View all Publications for this product

Customer reviews and Q&As

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Vinculin Staining

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Mouse Osteoblast cells were grown on cover slips for 48 hrs. Then Cells were fixed with formaldehyde vapor for 10 mins. Cells were permeabilized with Triton-X 100 for 5-7mins. 3% BSA was used for blocking (1hr). 1:100 dilution of rabbit anti-vinculin antibody to mouse was added onto the cells and incubated for 1hr. Then 1:1000 dilution of anti-rabbit FITC conjugated secondary antibody was added on to the cells and incubated for 1hr. Before proceeding to each step cells were washed with PBS.

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Verified customer

Submitted Jul 05 2013

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