Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rabbit
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Immunogen

    Full length protein. Rabbit IgG (containing the usual heavy and light chain)

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • Conjugation notes

    F/P ratio is 4.85
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Immunocytochemical/immunofluorescent analysis of  4% paraformaldehyde-fixed Triton X-100 permeabilized Goat skeletal muscle-derived satellite cells stained for NSE using ab53025.

    After washing in PBS, the cells were incubated with Goat Anti-Rabbit IgG H&L (FITC) (ab97050) (Green).

  • ab84235 staining melanoma inhibitory activity in a human melanoma cell line by Flow Cytometry. Cells were harvested with EDTA and washed in PBS. Cells were pemeabilized with saponine. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at 20°C. A FITC-conjugated goat anti-rabbit IgG H&L (ab97050) (1/100) was used as the secondary antibody.

     

    See Abreview

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3% PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness

     



    Primary antibodies:
    Mouse anti-Ki67, 1:100
    Rabbit anti-laminin alpha 1 (basement marker)

    Secondary antibodies:
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AMCA) (ab47052), 1:100
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050), 1:100

     

  • The FACS staining was perform on THP-1 cell lines with Rabbit polyclonal to CCR2 (ab21667) at a 1/100 dillution for 30 min at 4C. The secondary antibody was ab97050 used at 1/100 for 20min at 4C. The buffer used was PBS/BSA (0.5%)/Azide (0.05%). No fixation or permeabilization was performed and gating was done on alive cells.

    This image is courtesy of an anonymous Abreview

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rabbit anti-laminin alpha 1, 1:400
    Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050)
    F-actin was stained with CytoPainter F-actin staining kit (blue) (ab112124), 1:1000
    Nuclei were counterstained stained with DRAQ7TM (ab109202), 1:1000

  • The FACS staining was performed on HEK cells expressing human CCR5 with PBS/BSA (0.5%)/Azide (0.05%) as FACS buffer. We used a primary polyclonal rabbit antibody against CCR5 at a 1/100 dillution, incubated for 1 hour at 4C. The secondary antibody was ab97050 used at a concentration of 1/100 for 30min at 4C. No fixation or permeabilization was performed and gaiting was done on alive cells.

    This image is courtesy of an anonymous Abreview

References

This product has been referenced in:

  • Segunda MN  et al. Sertoli cell-mediated differentiation of bovine fetal mesenchymal stem cells into germ cell lineage using an in vitro co-culture system. Theriogenology 130:8-18 (2019). Read more (PubMed: 30852370) »
  • Chami B  et al. Serum Amyloid A Stimulates Vascular and Renal Dysfunction in Apolipoprotein E-Deficient Mice Fed a Normal Chow Diet. Front Immunol 10:380 (2019). Read more (PubMed: 30899260) »
See all 22 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.
According to the datasheet, the this protein is not expressed in most lymphoid cell lines: B-cells, T-cells and NK cells. Therefore, I would suggest to use a T-cell line like Jurkat lysate as a negative control.

https://www.abcam.com/index.html?datasheet=7899 https://www.abcam.com/index.html?datasheet=7899.

As a "negative control" for WB I woul suggest to perform a non-primary control, which simply means skipping the promary antibody incubation.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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Answer

Thank you for your email and interest in Abcam products. We currently do not have AlexaFluor conjugated antibodies or proteins available in our catalog. Having said this though, we have a number of products available that are conjugated to DyLight® fluorochromes. This family of dyes is comparable to AlexaFluor dyes regarding high fluorescence intensity, photostability and water solubility. Both the absorption and emission properties of the DyLight® fluorochomes are compatible with the excitation and detection wavelengths of common fluorescence instrumentation (https://www.abcam.com/DyLight). Please be reassured that all our products are covered by our Abpromise: We guarantee our products work in the tested species and applications as stated on the datasheet, and we will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase. Thus, please see a selection of our options: 1+2) Unfortunately, we currently do not have any phalloidin protein or antibody against phalloidin available. 3) ab96873, ab96881: Click here (or use the following: https://www.abcam.com/index.html?datasheet=96873). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96881). 4) ab96871, ab96871, ab97022: Click here (or use the following: https://www.abcam.com/index.html?datasheet=96871). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96871). Click here (or use the following: https://www.abcam.com/index.html?datasheet=97022). 5) ab102883, ab102884, ab102885 (please see https://www.abcam.com/EasyLink or attachment for more details): Click here (or use the following: https://www.abcam.com/index.html?datasheet=102883). Click here (or use the following: https://www.abcam.com/index.html?datasheet=102884). Click here (or use the following: https://www.abcam.com/index.html?datasheet=102885). 6) ab102923, ab1029234, ab102925 (please see https://www.abcam.com/EasyLink or attachment for more details): Click here (or use the following: https://www.abcam.com/index.html?datasheet=102923). Click here (or use the following: https://www.abcam.com/index.html?datasheet=102924). Click here (or use the following: https://www.abcam.com/index.html?datasheet=102925). 7) ab97050, ab96883, ab96899: Click here (or use the following: https://www.abcam.com/index.html?datasheet=97050). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96883). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96899). 8) ab96885, ab96897: Click here (or use the following: https://www.abcam.com/index.html?datasheet=96885). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96897). We also have the secondary antibodies in different formats (pre-absorbed, Fab fragments etc) which might be more suitable for certain applications. I will be happy to assist you in finding the most appropriate product on request. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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