Question (49364) | Goat Anti-Rabbit IgG H&L (FITC) (ab97050)

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According to the datasheet, the this protein is not expressed in most lymphoid cell lines: B-cells, T-cells and NK cells. Therefore, I would suggest to use a T-cell line like Jurkat lysate as a negative control.

https://www.abcam.com/Jurkat-whole-cell-lysate-ab7899.html https://www.abcam.com/Jurkat-whole-cell-lysate-ab7899.html.

As a "negative control" for WB I woul suggest to perform a non-primary control, which simply means skipping the promary antibody incubation.

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