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I have question which of negative control could you recommend for products: ab112538, ab6717, ab97050.
Asked on Jun 01 2012
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According to the datasheet, the this protein is not expressed in most lymphoid cell lines: B-cells, T-cells and NK cells. Therefore, I would suggest to use a T-cell line like Jurkat lysate as a negative control.
As a "negative control" for WB I woul suggest to perform a non-primary control, which simply means skipping the promary antibody incubation.
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Answered on Jun 01 2012