Goat Anti-Rabbit IgG H&L (FITC) preadsorbed (ab7086)

Abreviews (1)Q&A (2)Specific References (6)

Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (FITC) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Full length native Rabbit IgG (purified).

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Immunomicroscopy
Flow Cyt
IHC-P
IHC-Fr
ELISA
  • Application notes
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References

    This product has been referenced in:
    • Wang H  et al. Osteoblast-targeted delivery of miR-33-5p attenuates osteopenia development induced by mechanical unloading in mice. Cell Death Dis 9:170 (2018). ICC/IF . Read more (PubMed: 29415986) »
    • Li L  et al. Three-dimensional hepatocyte culture system for the study of Echinococcus multilocularis larval development. PLoS Negl Trop Dis 12:e0006309 (2018). Read more (PubMed: 29538424) »

    See all 6 Publications for this product

    Publishing research using ab7086? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    3 reviews or Q&A

    10
    Votes: Highest First

    Good secondary antibody

     Good
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Used as part of an ISH/IHC double stain on fresh frozen human skeletal muscle. Used at a 1/200 dilution to bind to the Abcam Caspase-3 primary antibody (ab52293).

    Incubated at room temperature for 60 minutes in a dark staining chamber.
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    Abcam user community

    Verified customer

    Submitted Aug 01 2016

    My customer is looking for an anti-mouse secondary antibody with fluorophore conjugate which can be detected with blue filters (wavelength 460-490 nm) in his fluorescence microscope. He is also considering ab7086 (anti-rabbit FITC) for a second primary...

    Read More

    Thank you for your inquiry.

    I can confirm that the ab47052 can be detected with this filter at 460 to 490nm. Indeed, the fluorochrome AMCA has a maximum emission at 445nm, however the fluorescent emission between 460 and 490nm should still be...

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    Our lab is about to start doing immunohistochemistry on frozen sections of mouse brain with primary antibody from rabbit. He have access to a confocal microscope and a fluorescent microscope with a UV lamp. Which antibody conjugation would allow for ...

    Read More

    Thank you for your enquiry. First, when selecting a secondary antibody conjugated to a fluorochrome, you're limited by the available microscope filter sets that go with your microscope. Most filter sets are best matched with rhodamine or fluorescein. T...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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