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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rabbit IgG H&L (FITC) preadsorbed (ab7086)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (12)

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Goat Anti-Rabbit IgG H&L (FITC) preadsorbed
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA

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Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (FITC) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    Full length native Rabbit IgG (purified).

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Fluorophore
    • FITC

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab7086 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    Immunomicroscopy
    Flow Cyt
    IHC-P
    IHC-Fr (1)
    ELISA
    Notes
  • Application notes
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (12)

    Publishing research using ab7086? Please let us know so that we can cite the reference in this datasheet.

    ab7086 has been referenced in 12 publications.

    • Amjadi F  et al. Comparative evaluation of NOTCH signaling molecules in the endometrium of women with various gynecological diseases during the window of implantation. Iran J Basic Med Sci 22:426-431 (2019). PubMed: 31168348
    • Wang X  et al. Elevated microRNA-145-5p increases matrix metalloproteinase-9 by activating the nuclear factor-?B pathway in rheumatoid arthritis. Mol Med Rep 20:2703-2711 (2019). PubMed: 31322192
    • Zhuang L  et al. Co-culturing with hypoxia pre-conditioned mesenchymal stem cells as a new strategy for the prevention of irradiation-induced fibroblast-to-myofibroblast transition. Oncol Rep 42:1781-1792 (2019). PubMed: 31485596
    • Cao X  et al. Y-box binding protein 1 regulates ox-LDL mediated inflammatory responses and lipid uptake in macrophages. Free Radic Biol Med 141:10-20 (2019). PubMed: 31153975
    • Cui L  et al. miR-194 suppresses epithelial-mesenchymal transition of retinal pigment epithelial cells by directly targeting ZEB1. Ann Transl Med 7:751 (2019). PubMed: 32042767
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Good secondary antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Used as part of an ISH/IHC double stain on fresh frozen human skeletal muscle. Used at a 1/200 dilution to bind to the Abcam Caspase-3 primary antibody (ab52293).

    Incubated at room temperature for 60 minutes in a dark staining chamber.

    Abcam user community

    Verified customer

    Submitted Aug 01 2016

    Question

    My customer is looking for an anti-mouse secondary antibody with fluorophore conjugate which can be detected with blue filters (wavelength 460-490 nm) in his fluorescence microscope. He is also considering ab7086 (anti-rabbit FITC) for a second primary antibody for his double IF simultaneous protocol.

    Please advise if ab47052 can be detected with this blue filter? If not, can you kindly recommend one?

    Thank you.

    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 19 2012

    Answer

    Thank you for your inquiry.

    I can confirm that the ab47052 can be detected with this filter at 460 to 490nm. Indeed, the fluorochrome AMCA has a maximum emission at 445nm, however the fluorescent emission between 460 and 490nm should still be detectable. Obviously the signal would however be stronger if the customer would have a filter letting pass 445nm as well. Please note however that the ab47052 is an anti mouse antibody.

    The ab7086 coupled to FITC can also be used as emission maximum of FITC is at 519nm. Even though the spectrum is quite large, the emissionis very low under 500nm. (http://en.wikipedia.org/wiki/Fluorescein)I am therefore positive that those two fluorochromes can be used together without any significant interference.

    Here is also a link to the our guide to fluorescent dyes: https://docs.abcam.com/pdf/immunology/fluorochrome_guide.pdf

    The only thing I would like to draw your attention to, is that the ab47052 has not been crossabsorbed. This means we do not know whether this antibody would also recognize the rabbit antibodies. For a double staining it is therefore imperative that the customer makes this control (using only primary rabbit antibody and secondary anti mouse antibody to see whether there is any crossreaction). We unfortunately do not have another anti mouse antibody with a "blue" fluorochrome which would be already pre-absorbed. The ab7086 howeverhas been preabsorbed and is therefore perfectly fine for the double staining.

    I hope this information is helpful. Please do not hesitate to get back to us, should you have any additional questions.

    Read More

    Abcam Scientific Support

    Answered on Jul 19 2012

    Question

    Our lab is about to start doing immunohistochemistry on frozen sections of mouse brain with primary antibody from rabbit. He have access to a confocal microscope and a fluorescent microscope with a UV lamp. Which antibody conjugation would allow for the easiest and best signal detection (ab7086, ab6720, etc.)? Also, I have rviewed your protocols for IHC, and find all the various adjustments confusing. If you can make a recommendation, can you also please indicate which protocol should be followed? Thank you.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 24 2005

    Answer

    Thank you for your enquiry. First, when selecting a secondary antibody conjugated to a fluorochrome, you're limited by the available microscope filter sets that go with your microscope. Most filter sets are best matched with rhodamine or fluorescein. Texas Red® may also be used with a rhodamine filter set. So you want to determine which filter sets that you have and then choose an appropriate secondary to go along with your primary antibody. On our website we have a nice chart with various fluorochromes and their corresponding wavelengths. Just select the resources tab and then click on antibody guide from the drop-down menu. Then choose "choosing an antibody" and click go. I would recommend following the 2 Step Fluorescent protocol that we have listed on the IHC - Frozen sections / Cytological preparations protocols section on our website. A really good IHC website (IHC World) that you may want to refer to is below. http://www.ihcworld.com/introduction.htm It has a lot of good protocols and details, and should be helpful for you. If you have any additional questions, please do contact us again.

    Read More

    Abcam Scientific Support

    Answered on Jun 28 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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