Product nameGoat Anti-Rabbit IgG H&L (Glucose Oxidase)
See all IgG secondary antibodies
Tested applicationsSuitable for: ELISA, WB, ICC, IHC-P, IHC-Frmore details
HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Highly purified whole rabbit immunoglobulin.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituents: 50% Glycerol, 50% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab136782 has been purified using a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
Our Abpromise guarantee covers the use of ab136782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/100 - 1/1000. Substrate: 10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm.|
|ICC||1/10 - 1/100. Substrate: 15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.|
|IHC-P||1/10 - 1/100.|
|IHC-Fr||1/10 - 1/100.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab136782 has not yet been referenced specifically in any publications.