Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab136636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/10000.
WB 1/1000 - 1/5000.
IP 1/5000 - 1/30000.

References

This product has been referenced in:

  • Yang C  et al. Platelet-derived growth factor-D expression mediates the effect of differentiated degree on prognosis in epithelial ovarian cancer. J Cell Biochem N/A:N/A (2019). Read more (PubMed: 30652340) »
  • Rajan A  et al. A Mechanism Coupling Systemic Energy Sensing to Adipokine Secretion. Dev Cell 43:83-98.e6 (2017). Read more (PubMed: 29017032) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting Abcam.

The main difference in the 3 secondary antibodies you mentioned are in the applications that they are tested and guaranteed to work in for 6 months as stated in our AbPromise guarantee, the method in which they were purified, and whether or not they are labeled as our "AbExcel" antibodies. Below I summarize these differences for you:

ab6721 (https://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-hl-hrp-ab6721.html)
Tested applications: IHC-P, WB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-Fr
Purification method: IgG fraction
AbExcel: No

ab97051 (https://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-hl-hrp-ab97051.html)
Tested applications: ICC, IHC-P, ELISA, WB
Purification method: Immunogen affinity purified
AbExcel: Yes (description of AbExcel: https://www.abcam.com/index.html?pageconfig=resource&rid=13534)

ab136636 (https://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-hrp-ab136636.html)
Tested applications: ELISA, WB, IP
Purification method: Unlisted
AbExcel: No

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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