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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  • Datasheet
  • SDS
Reviews (8)Q&A (14)References (2075)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
  • Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

Key features and details

  • Goat Anti-Rabbit IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC, IHC-Fr

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Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC, IHC-Frmore details
  • Immunogen

    Rabbit IgG, whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section. Store In the Dark.
  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • Conjugation notes

    Horseradish Peroxidase (HRP)
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    HRP conjugated anti-rabbit secondary antibody optimized for western blot and immunohistochemistry. Some customers reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.

    Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

     

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Enzyme
    • HRP

Associated products

  • Related Products

    • Normal Goat Serum (ab7481)
  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
1/1000.
WB (6)
1/2000 - 1/20000.

Suggested working dilution of 1/3000 (see PMID: 17222046). In addition, found to work at 1/20000 (see PMID: 16936283).

Working dilutions are highlighted in the table below. Please note that the antibody can be diluted to 1:48,000 to 1:207,000 in many instances.

ELISA
1/120000.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC
1/1000 - 1/5000.
IHC-Fr
1/1000.
Notes
IHC-P
1/1000.
WB
1/2000 - 1/20000.

Suggested working dilution of 1/3000 (see PMID: 17222046). In addition, found to work at 1/20000 (see PMID: 16936283).

Working dilutions are highlighted in the table below. Please note that the antibody can be diluted to 1:48,000 to 1:207,000 in many instances.

ELISA
1/120000.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC
1/1000 - 1/5000.
IHC-Fr
1/1000.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)Farah et al PLoS One. 2018 Feb 2;13(2):e0191526. doi: 10.1371/journal.pone.0191526. eCollection 2018. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Anti-phospho-tau immunostaining in Dryocopus lineatus.

    Tau-positivity in the midbrain (Panel A (shown) and B) and the corpus callosum (C) of the Dryocopus lineatus brain. The axonal tract staining demonstrates a thread-like pattern, similar to that seen with Gallyas sliver staining. Occasional intracellular tau-accumulations were identified within neurons (D).

    For full method, please see paper.

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)This image is courtesy of an Abreview submitted by Maryna Polyakova.
    All lanes : Anti-NEK7 antibody (ab80948) at 1/2000 dilution

    All lanes : Mouse brain tissue cytoplasmic lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds


    Blocked with 5% non-fat milk for 1 hour at 18°C

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)This image is courtesy of an anonymous Abreview.

    ab3580 staining glucocorticoid receptor in mouse epididymis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with Bouin's solution and blocked with 1.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000) for 14 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG H&L (ab6721) (1/200) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)Image is courtesy of an anonymous AbReview.

    Immunohistochemical analysis of PFA-fixed paraffin-embedded rat cardiac tissue sections, labeling Conexin 43 with ab117843 at a dilution of 1/500 incubated for 12 hours at 4°C in 1% BSA in TBS. Antigen retrival was via Tris-EDTA pH 9.0 (heat mediated). Blocking was 3% BSA incubated for 1 hour at 37°C. The secondary was ab6721 at 1/500.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

    ab6721 was used at dilution 1/100 with the primary antibody ab11370 in IHC-P. See the review on ab11370.

  • Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
    Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

    ab6721 was used at dilution 1/100 with the primary antibody ab35604 in IHC-Fr. See the review on ab35604.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2075)

Publishing research using ab6721? Please let us know so that we can cite the reference in this datasheet.

ab6721 has been referenced in 2075 publications.

  • Gao Z  et al. Regulatory effects of lncRNA ATB targeting miR-200c on proliferation and apoptosis of colorectal cancer cells. J Cell Biochem 121:332-343 (2020). PubMed: 31222825
  • Li L  et al. Brivanib, a multitargeted small-molecule tyrosine kinase inhibitor, suppresses laser-induced CNV in a mouse model of neovascular AMD. J Cell Physiol 235:1259-1273 (2020). PubMed: 31270802
  • Pan SC  et al. Preliminary Assessment of Burn Depth by Paper-Based ELISA for the Detection of Angiogenin in Burn Blister Fluid-A Proof of Concept. Diagnostics (Basel) 10:N/A (2020). ELISA ; Human . PubMed: 32120826
  • Guo Z  et al. Cordycepin suppresses the migration and invasion of human liver cancer cells by downregulating the expression of CXCR4. Int J Mol Med 45:141-150 (2020). PubMed: 31746344
  • Pan S  et al. S100A8 facilitates cholangiocarcinoma metastasis via upregulation of VEGF through TLR4/NF-?B pathway activation. Int J Oncol 56:101-112 (2020). PubMed: 31746424
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review

Filter by Application

Filter by Ratings

1-8 of 8 Abreviews

EXCELLENT SECONDARY ANTIBODY for WB

Excellent
Abreviews
Abreviews
Application
Western blot
EXCELLENT SECONDARY ANTIBODY for WB

Mrs. Kyunga Kwak

Verified customer

Submitted May 07 2020

HRP for western blotting assay

Good
Abreviews
Abreviews
Application
Western blot
I have been using this secondary antibody for my western blotting experiments and its working fine with all of proteins of our interest (i.e. Rodent CYP-450 enzymes like CYP-3A, 2D1, 2E1, 1A2, 2B1 etc.)
Before using this I had also explored the HRP antibody from other vendors but they were not so specific I was having lot of back ground noise on my membranes irrespective of varying dilutions and incubation times and so on.
So I would highly recommend using this for western blot experiments.

Abcam user community

Verified customer

Submitted Nov 20 2019

WB against mRFP (R) protein

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
The antibody works very well, detects well two different protein tagged with mRFP.

Abcam user community

Verified customer

Submitted Apr 30 2019

Very sensitive and specific secondary antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
The dilution used for this secondary antibody was 1/20000 and we obtained intesnse specific bands even at very short incubation times. It works very well with phosphorylated proteins which sometimes are harder to detect (in these cases we use 1/10000 dilution or 1/5000).

Abcam user community

Verified customer

Submitted Oct 03 2018

ab6721

Excellent
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
This HRP-conjugated antibody works very well.

Dr. Shyambabu Chaurasiya

Verified customer

Submitted Jan 24 2018

Immunohistochemistry-Abreview for PFA fixed, paraffin embedded Goat anti-Rabbit HRP secondary antibody ab6721

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Very clear signal. The image is of a cross section of 3 metatarsal bones, primary antibody is Periostin (ab14041).

Haydee Gutierrez

Verified customer

Submitted Jan 09 2017

EXCELLENT SECONDARY ANTIBODY

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Secondary antibody (ab6721) was used at a dilution of 1:5000 in 5% BSA. Placental lysate was used. primary antibody was purchased for VEGFR1 (ab 32152) antibody.
gel: 10%, 200 V for 35 min.
transfer 17 V for 35 min. using Biorad TURBO
Blocking 5% BSA.

Vicky

Verified customer

Submitted Nov 07 2016

Western blot - Goat Anti-Rabbit IgG H&L (HRP) for detection of Rabbit Glutathione Peroxidase primary AB in bone (tibia) tissue lysate

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Experimental conditions:
Bone tissue lysate (tibia) extracted with RIPA buffer
10% SDS-PAGE
Blocking 1h with 5% milk in TBS-T at RT
Incubation with primary AB 1:1000 overnight at 4ºC
Incubation with secondary AB (ab6721 - Goat Anti-Rabbit IgG) 1:2000 for 1h at RT
Developed with ECL for 537s
Results
No nonspecific binding. No background. Clear bands.

Mr. Helder Fonseca

Verified customer

Submitted Oct 07 2014

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