Application
Western blot
dilution buffer: 5% non-fat milk in PBS-Tween
dilution: 1/20000
incubation time: 1hour
exposure time: 30sec
Figure legend:
Western blot for HURP and alpha tubulin. MDA-MB-436 cells transfected with 50nM siRNA against HURP protein(lane 1) or 50nM non-targeting siRNA(lane 2) for 72hrs.
dilution: 1/20000
incubation time: 1hour
exposure time: 30sec
Figure legend:
Western blot for HURP and alpha tubulin. MDA-MB-436 cells transfected with 50nM siRNA against HURP protein(lane 1) or 50nM non-targeting siRNA(lane 2) for 72hrs.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Christos Efstathiou
Verified customer
Submitted Mar 09 2022