Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)
- Datasheet
- References (5)
- Protocols
Overview
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Product name
Goat Anti-Rabbit IgG H&L (HRP polymer)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
Based on IEP, ab214880 reacts with heavy (gamma) chains on rabbit IgG, light chains on all rabbit immunoglobulins. No cross-reactivity is observed to non-immunoglobulin rabbit serum proteins, serum proteins from human, mouse or rat. -
Tested applications
Suitable for: IHC-P, Immunoelectrophoresismore details -
Immunogen
Other Immunogen Type corresponding to Rabbit IgG. (Purified Rabbit IgG, whole molecule).
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Conjugation
HRP polymer
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Proclin
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
ab214880 was affinity purified using solid phase Rabbit IgG. Purity >95% by SDS-PAGE. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
Our Abpromise guarantee covers the use of ab214880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| Immunoelectrophoresis | Use at an assay dependent concentration. This product is provided in a pre-diluted, ready-to-use format. Incubate 150 µl per slide for 1 hour. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)Sonzogni et al PLoS Biol. 2018 Jun 20;16(6):e2004049. doi: 10.1371/journal.pbio.2004049. eCollection 2018 Jun. Fig S2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Representative images IHC for Ki67 (upper panel) and CC3 (lower panel); scale bar 50 μm.
The paraffin sections were deparaffinized and rehydrated, followed by antigen retrieval using sodium citrate buffer (pH 6). The sections were then incubated with 3% hydrogen peroxide to block endogenous peroxidase activity for 10 minutes at room temperature. After 3 washes with TBS, the sections were incubated with 2% bovine serum albumin for 1 hour at room temperature.
Slides were then incubated separately with rabbit anti-Ki67 and rabbit anti-cleaved caspase 3 overnight at 4°C. The slides were washed 3 times and incubated with goat anti-rabbit HRP polymer (1:1, Abcam ab214880) for 2 hours at room temperature. Samples were washed thrice and then developed, counterstained with hematoxylin and then dehydrated before mounting the slides with permount.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)Immunohistochemical analysis of ER/PR positive breast cancer tissue stained for ER and detected with ab214880.
References
This product has been referenced in:
- Sonzogni O et al. Reporters to mark and eliminate basal or luminal epithelial cells in culture and in vivo. PLoS Biol 16:e2004049 (2018). Read more (PubMed: 29924804) »
- Luo L et al. Bletilla striata polysaccharide has a protective effect on intestinal epithelial barrier disruption in TAA-induced cirrhotic rats. Exp Ther Med 16:1715-1722 (2018). Read more (PubMed: 30186392) »
