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  1. Link

    goat-rabbit-igg-hl-hrp-polymer-ab214880.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (9)

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

Key features and details

  • Goat Anti-Rabbit IgG H&L (HRP polymer)
  • Conjugation: HRP polymer
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, Immunoelectrophoresis

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Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (HRP polymer)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rabbit
  • Specificity

    Based on IEP, ab214880 reacts with heavy (gamma) chains on rabbit IgG, light chains on all rabbit immunoglobulins. No cross-reactivity is observed to non-immunoglobulin rabbit serum proteins, serum proteins from human, mouse or rat.
  • Tested applications

    Suitable for: IHC-P, Immunoelectrophoresismore details
  • Immunogen

    Other Immunogen Type corresponding to Rabbit IgG. (Purified Rabbit IgG, whole molecule).

  • Conjugation

    HRP polymer

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% CMIT/MIT 3:1
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    ab214880 was affinity purified using solid phase Rabbit IgG. Purity >95% by SDS-PAGE.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Enzyme
    • HRP

Associated products

    Applications

    Our Abpromise guarantee covers the use of ab214880 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use at an assay dependent concentration.
    Immunoelectrophoresis Use at an assay dependent concentration.

    This product is provided in a pre-diluted, ready-to-use format. Incubate 150 µl per slide for 1 hour.

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)Sonzogni et al PLoS Biol. 2018 Jun 20;16(6):e2004049. doi: 10.1371/journal.pbio.2004049. eCollection 2018 Jun. Fig S2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Representative images IHC for Ki67 (upper panel) and CC3 (lower panel); scale bar 50 μm.

      The paraffin sections were deparaffinized and rehydrated, followed by antigen retrieval using sodium citrate buffer (pH 6). The sections were then incubated with 3% hydrogen peroxide to block endogenous peroxidase activity for 10 minutes at room temperature. After 3 washes with TBS, the sections were incubated with 2% bovine serum albumin for 1 hour at room temperature.

      Slides were then incubated separately with rabbit anti-Ki67 and rabbit anti-cleaved caspase 3 overnight at 4°C. The slides were washed 3 times and incubated with goat anti-rabbit HRP polymer (1:1, Abcam ab214880) for 2 hours at room temperature. Samples were washed thrice and then developed, counterstained with hematoxylin and then dehydrated before mounting the slides with permount.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

      Immunohistochemical analysis of ER/PR positive breast cancer tissue stained for ER and detected with ab214880.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (9)

    Publishing research using ab214880? Please let us know so that we can cite the reference in this datasheet.

    ab214880 has been referenced in 9 publications.

    • Zhao C  et al. The Renoprotective Effects of Naringenin (NGN) in Gestational Pregnancy. Diabetes Metab Syndr Obes 13:53-63 (2020). PubMed: 32021351
    • Yang Y  et al. Mechanism of cell death induced by silica nanoparticles in hepatocyte cells is by apoptosis. Int J Mol Med 44:903-912 (2019). PubMed: 31524225
    • Qiu Z  et al. miR-370 inhibits the oxidative stress and apoptosis of cardiac myocytes induced by hydrogen peroxide by targeting FOXO1. Exp Ther Med 18:3025-3031 (2019). PubMed: 31555385
    • Sonzogni O  et al. Reporters to mark and eliminate basal or luminal epithelial cells in culture and in vivo. PLoS Biol 16:e2004049 (2018). PubMed: 29924804
    • Luo L  et al. Bletilla striata polysaccharide has a protective effect on intestinal epithelial barrier disruption in TAA-induced cirrhotic rats. Exp Ther Med 16:1715-1722 (2018). PubMed: 30186392
    • Zhang C  et al. FOXA1 is upregulated in glioma and promotes proliferation as well as cell cycle through regulation of cyclin D1 expression. Cancer Manag Res 10:3283-3293 (2018). PubMed: 30233244
    • Li F  et al. Blocking the CD47-SIRPa axis by delivery of anti-CD47 antibody induces antitumor effects in glioma and glioma stem cells. Oncoimmunology 7:e1391973 (2018). PubMed: 29308321
    • Nakashima H  et al. Toxicity and Efficacy of a Novel GADD34-expressing Oncolytic HSV-1 for the Treatment of Experimental Glioblastoma. Clin Cancer Res 24:2574-2584 (2018). PubMed: 29511029
    • Fang L  et al. Resistance to epithelial growth factor receptor tyrosine kinase inhibitors in a patient with transformation from lung adenocarcinoma to small cell lung cancer: A case report. Oncol Lett 14:593-598 (2017). PubMed: 28693210

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    IHC staining of Ki-67 in FFPE human tumor using Goat anti-Rabbit IgG (HRP polymer)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval: 10mM Citrate pH6, boil for 10 minutes in pressure cooker
    Primary antibody: Rabbit anti-67, incubate overnight at 4oC
    Blocking and antibody diluent: 2% BSA in PBS
    Secondary antibody: Goat anti-Rabbit IgG H&L )HRP polymer) used at 2x dilution, incubate at room temperature for 90 minutes
    DAB detection: DAB detection kit from Vector lab
    Counterstain with Hematoxylin (Blue)

    Abcam user community

    Verified customer

    Submitted Dec 14 2017

    IHC staining of Ki-67 in FFPE pig lung using Goat anti-rabbit IgG (HRP polymer)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval: 10mM Sodium Citrate pH 6, boiling for 10 minutes in pressure cooker
    Primary antibody: Rabbit anti-Ki-67, overnight incubation at 4oC
    Blocking reagent and Antibody diluent: 2% BSA in PBS
    Secondary antibody: Goat anti-Rabbit IgG H&L (HRP polymer) used at 5x dilution in 2% BSA for 90 minutes at room temperature
    DAB detection: DAB detection kit from Vector lab
    Hematoxylin counterstain: blue

    Abcam user community

    Verified customer

    Submitted Dec 12 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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