Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)
Key features and details
- Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
- Conjugation: IRDye® 680RD
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, In-Cell ELISA
Related conjugates and formulations
Overview
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Product name
Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Tested applications
Suitable for: WB, In-Cell ELISAmore details -
Minimal
cross-reactivity
Chicken, Human, Mouse, Rat, Sheep more details -
Immunogen
Full length native protein (purified) corresponding to Rabbit IgG.
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Conjugation
IRDye® 680RD
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS
Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer. -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Affinity chromatography purified using pooled IgG covalently linked to agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000 - 1/25000.
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| In-Cell ELISA |
1/200 - 1/800.
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| Notes |
|---|
|
WB
1/5000 - 1/25000. |
|
In-Cell ELISA
1/200 - 1/800. |
Images
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Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)All lanes : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) at 1/10000 dilution (Anti-GAPDH antibody)
Lane 1 : Wild-type HAP1 cell lysate (20 µg)
Lane 2 : Beta actin knockout HAP1 cell lysate (20 µg)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution (Goat anti-Mouse IgG H&L (IRDye® 680RD))This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with Anti-GAPDH antibody ab181602 overnight at 4°C. Antibody binding was detected using the Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Image shows merged signal (red and green). Green - Anti-beta Actin antibody [AC-15] (ab6276)ab6276 observed at 42 kDa. Red - GAPDH loading control, ab181602, observed at 37 kDa.
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In-Cell ELISA - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)Image taken from In-cell ELISA PhosphoPDH Kit (ab110218), which uses Goat anti-mouse IgG H&L (IRDye® 680RD) preadsorbed ab216777 for detection among its components. -
Spectrum - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)Excitation and emission spectra of IRDye® 680RD Ex: 680, Em:694.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab216777 has been referenced in 8 publications.
- Zhou Y et al. lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR-500a-3p/XBP-1 axis. Mol Med Rep 24:N/A (2021). PubMed: 33982777
- Li Y et al. REDD1 (regulated in development and DNA damage-1)/autophagy inhibition ameliorates fine particulate matter (PM2.5) -induced inflammation and apoptosis in BEAS-2B cells. Bioengineered 12:1403-1414 (2021). PubMed: 33926343
- Polaski JT et al. The origins and consequences of UPF1 variants in pancreatic adenosquamous carcinoma. Elife 10:N/A (2021). PubMed: 33404013
- Chien HC et al. PPARd and FOXO1 Mediate Palmitate-Induced Inhibition of Muscle Pyruvate Dehydrogenase Complex and CHO Oxidation, Events Reversed by Electrical Pulse Stimulation. Int J Mol Sci 21:N/A (2020). PubMed: 32824862
- Li Z et al. TRIM21-mediated proteasomal degradation of SAMHD1 regulates its antiviral activity. EMBO Rep 21:e47528 (2020). PubMed: 31797533
- Peacock TP et al. Swine ANP32A Supports Avian Influenza Virus Polymerase. J Virol 94:N/A (2020). PubMed: 32269123
- Sun K et al. Postretrieval Microinjection of Baclofen Into the Agranular Insular Cortex Inhibits Morphine-Induced CPP by Disrupting Reconsolidation. Front Pharmacol 11:743 (2020). PubMed: 32508658
- Elbaz-Alon Y et al. PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria. Nat Commun 11:3645 (2020). PubMed: 32686675