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  1. Link

    goat-rabbit-igg-hl-irdye-680rd-preadsorbed-ab216777.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)

  • Datasheet
  • SDS
Submit a review Submit a question References (8)

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Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)
  • In-Cell ELISA - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)
  • Spectrum - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)

Key features and details

  • Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
  • Conjugation: IRDye® 680RD
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: WB, In-Cell ELISA

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy3.5 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 12nm Gold 6nm HRP HRP polymer IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated Unconjugated

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Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rabbit
  • Tested applications

    Suitable for: WB, In-Cell ELISAmore details
  • Minimal
    cross-reactivity


    Chicken, Human, Mouse, Rat, Sheep
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    Full length native protein (purified) corresponding to Rabbit IgG.

  • Conjugation

    IRDye® 680RD

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: PBS

    Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer.
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Affinity chromatography purified using pooled IgG covalently linked to agarose.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG

Associated products

  • Related Products

    • Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab216777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000 - 1/25000.
In-Cell ELISA
1/200 - 1/800.
Notes
WB
1/5000 - 1/25000.
In-Cell ELISA
1/200 - 1/800.

Images

  • Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)
    Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)
    All lanes : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) at 1/10000 dilution (Anti-GAPDH antibody)

    Lane 1 : Wild-type HAP1 cell lysate (20 µg)
    Lane 2 : Beta actin knockout HAP1 cell lysate (20 µg)

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution (Goat anti-Mouse IgG H&L (IRDye® 680RD))


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with Anti-GAPDH antibody ab181602 overnight at 4°C. Antibody binding was detected using the Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    Image shows merged signal (red and green). Green - Anti-beta Actin antibody [AC-15] (ab6276)ab6276 observed at 42 kDa. Red - GAPDH loading control, ab181602, observed at 37 kDa.

  • In-Cell ELISA - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)
    In-Cell ELISA - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)
    Image taken from In-cell ELISA PhosphoPDH Kit (ab110218), which uses Goat anti-mouse IgG H&L (IRDye® 680RD) preadsorbed ab216777 for detection among its components.
  • Spectrum - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)
    Spectrum - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)

    Excitation and emission spectra of IRDye® 680RD Ex: 680, Em:694.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (8)

Publishing research using ab216777? Please let us know so that we can cite the reference in this datasheet.

ab216777 has been referenced in 8 publications.

  • Zhou Y  et al. lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR-500a-3p/XBP-1 axis. Mol Med Rep 24:N/A (2021). PubMed: 33982777
  • Li Y  et al. REDD1 (regulated in development and DNA damage-1)/autophagy inhibition ameliorates fine particulate matter (PM2.5) -induced inflammation and apoptosis in BEAS-2B cells. Bioengineered 12:1403-1414 (2021). PubMed: 33926343
  • Polaski JT  et al. The origins and consequences of UPF1 variants in pancreatic adenosquamous carcinoma. Elife 10:N/A (2021). PubMed: 33404013
  • Chien HC  et al. PPARd and FOXO1 Mediate Palmitate-Induced Inhibition of Muscle Pyruvate Dehydrogenase Complex and CHO Oxidation, Events Reversed by Electrical Pulse Stimulation. Int J Mol Sci 21:N/A (2020). PubMed: 32824862
  • Li Z  et al. TRIM21-mediated proteasomal degradation of SAMHD1 regulates its antiviral activity. EMBO Rep 21:e47528 (2020). PubMed: 31797533
  • Peacock TP  et al. Swine ANP32A Supports Avian Influenza Virus Polymerase. J Virol 94:N/A (2020). PubMed: 32269123
  • Sun K  et al. Postretrieval Microinjection of Baclofen Into the Agranular Insular Cortex Inhibits Morphine-Induced CPP by Disrupting Reconsolidation. Front Pharmacol 11:743 (2020). PubMed: 32508658
  • Elbaz-Alon Y  et al. PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria. Nat Commun 11:3645 (2020). PubMed: 32686675

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