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Immunology Immunoglobulins Heavy Chain IgG
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Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (13)

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Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Spectrum - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)

Key features and details

  • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed
  • Conjugation: IRDye® 800CW
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: WB, In-Cell ELISA

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Overview

  • Product name

    Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed
    See all IgG secondary antibodies
  • Description

    Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed
  • Host species

    Goat
  • Target species

    Rabbit
  • Tested applications

    Suitable for: WB, In-Cell ELISAmore details
  • Minimal
    cross-reactivity


    Chicken, Human, Mouse, Rat, Sheep
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    Full length protein corresponding to Rabbit IgG.

  • Conjugation

    IRDye® 800CW

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: PBS

    Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer.
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Affinity chromatography purified using pooled rabbit IgG covalently linked to agarose.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab216773 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/5000 - 1/25000.
    In-Cell ELISA (1)
    1/200 - 1/1200.
    Notes
    WB
    1/5000 - 1/25000.
    In-Cell ELISA
    1/200 - 1/1200.

    Images

    • Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
      Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
      All lanes : Anti-NF-kB p65 antibody (ab16502) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 cell lysate (20 µg)
      Lane 2 : NF-kB p65 knockout HAP1 cell lysate (20 µg)
      Lane 3 : HeLa cell lysate (20 µg)
      Lane 4 : A431 cell lysate (20 µg)

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution (Goat anti-Rabbit IgG H&L (IRDye® 800CW))


      This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab16502 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

      Merged signal (red and green). Green - ab16502 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776.

    • Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
      Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
      All lanes : Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1 µg/ml (Anti-Lamin B1 antibody - Nuclear Envelope Marker)

      Lane 1 : Wild-type HAP1 nuclear lysate, 10 ug
      Lane 2 : Lamin B1 knockout HAP1 nuclear lysate

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution


      This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab16048 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

      Merged signal (red and green).  Green signal from target - ab16048 observed at 68 kDa. Red signal from loading control - ab10799 ( (loading control to histone H3 at 0.1µg/ml) observed at 18 kDa using as secondary Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776.

    • Spectrum - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
      Spectrum - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)

      Excitation and emission spectrum of IRDye® 800CW Ex: 776nm, Em: 792nm.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (13)

    Publishing research using ab216773? Please let us know so that we can cite the reference in this datasheet.

    ab216773 has been referenced in 13 publications.

    • Tian X  et al. Long noncoding RNA LINC00662 promotes M2 macrophage polarization and hepatocellular carcinoma progression via activating Wnt/ß-catenin signaling. Mol Oncol 14:462-483 (2020). PubMed: 31785055
    • Piers TM  et al. A locked immunometabolic switch underlies TREM2 R47H loss of function in human iPSC-derived microglia. FASEB J 34:2436-2450 (2020). PubMed: 31907987
    • Tatebe H  et al. Rad50 zinc hook functions as a constitutive dimerization module interchangeable with SMC hinge. Nat Commun 11:370 (2020). PubMed: 31953386
    • Schukken KM  et al. Altering microtubule dynamics is synergistically toxic with spindle assembly checkpoint inhibition. Life Sci Alliance 3:N/A (2020). PubMed: 31980556
    • Cao H  et al. miR-145-5p Regulates the Proliferation, Migration and Invasion in Cervical Carcinoma by Targeting KLF5. Onco Targets Ther 13:2369-2376 (2020). PubMed: 32256087
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    In cell ELISA for different primary antibodies

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    In-Cell ELISA
    We tested this product for in cell ELISA for antibody validation, and found it worked well.
    We used 6 different rabbit antibodies against the same target and performed in cell ELISA with wild type Hela cells and two different CRISPR mediated knockout cells as positive and negative controls, respectively. After staining with primary antibodies, cells were stained with x10,000 diluted this product and analyzed by using Odyssey CLx (LI-COR).

    Abcam user community

    Verified customer

    Submitted Sep 20 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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