Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
Key features and details
- Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed
- Conjugation: IRDye® 800CW
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, In-Cell ELISA
Overview
-
Product name
Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed
See all IgG secondary antibodies -
Description
Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed -
Host species
Goat -
Target species
Rabbit -
Tested applications
Suitable for: WB, In-Cell ELISAmore details -
Minimal
cross-reactivity
Chicken, Human, Mouse, Rat, Sheep more details -
Immunogen
Full length protein corresponding to Rabbit IgG.
-
Conjugation
IRDye® 800CW
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS
Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer. -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Affinity chromatography purified using pooled rabbit IgG covalently linked to agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000 - 1/25000.
|
|
| In-Cell ELISA | (1) |
1/200 - 1/1200.
|
| Notes |
|---|
|
WB
1/5000 - 1/25000. |
|
In-Cell ELISA
1/200 - 1/1200. |
Images
-
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)All lanes : Anti-NF-kB p65 antibody (ab16502) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate (20 µg)
Lane 2 : NF-kB p65 knockout HAP1 cell lysate (20 µg)
Lane 3 : HeLa cell lysate (20 µg)
Lane 4 : A431 cell lysate (20 µg)
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution (Goat anti-Rabbit IgG H&L (IRDye® 800CW))This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab16502 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Merged signal (red and green). Green - ab16502 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776.
-
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)All lanes : Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1 µg/ml (Anti-Lamin B1 antibody - Nuclear Envelope Marker)
Lane 1 : Wild-type HAP1 nuclear lysate, 10 ug
Lane 2 : Lamin B1 knockout HAP1 nuclear lysate
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilutionThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab16048 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Merged signal (red and green). Green signal from target - ab16048 observed at 68 kDa. Red signal from loading control - ab10799 ( (loading control to histone H3 at 0.1µg/ml) observed at 18 kDa using as secondary Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776.
-
Spectrum - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)Excitation and emission spectrum of IRDye® 800CW Ex: 776nm, Em: 792nm.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (13)
ab216773 has been referenced in 13 publications.
- Tian X et al. Long noncoding RNA LINC00662 promotes M2 macrophage polarization and hepatocellular carcinoma progression via activating Wnt/ß-catenin signaling. Mol Oncol 14:462-483 (2020). PubMed: 31785055
- Piers TM et al. A locked immunometabolic switch underlies TREM2 R47H loss of function in human iPSC-derived microglia. FASEB J 34:2436-2450 (2020). PubMed: 31907987
- Tatebe H et al. Rad50 zinc hook functions as a constitutive dimerization module interchangeable with SMC hinge. Nat Commun 11:370 (2020). PubMed: 31953386
- Schukken KM et al. Altering microtubule dynamics is synergistically toxic with spindle assembly checkpoint inhibition. Life Sci Alliance 3:N/A (2020). PubMed: 31980556
- Cao H et al. miR-145-5p Regulates the Proliferation, Migration and Invasion in Cervical Carcinoma by Targeting KLF5. Onco Targets Ther 13:2369-2376 (2020). PubMed: 32256087
