Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)

Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed
    See all IgG secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Rabbit IgG (PE), pre-adsorbed
  • Host species

    Goat
  • Target species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity


    Cow, Mouse more details
  • Immunogen

    Rabbit IgG

  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1.64% Sodium phosphate, 1.71% Sucrose, 0.87% Sodium chloride, 0.1% BSA
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.
  • Conjugation notes

    Excitation max. l 565>498 nm. Emission max. l 578 nm. F/P 0.8:1.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab72465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500 - 1/1000.
ELISA Use at an assay dependent concentration.

Images

  • Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Goat anti-rabbit IgG H&L (Phycoerythrin, pre-adsorbed) (ab72465) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 575/30 bandpass filter.

  • Sandwich ELISA to detect Rabbit IgG. Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated Rabbit IgG and read after a 2 hour incubation at room temperature with ab72465

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for contacting Abcam.

This product has not been tested in ICC and so while we cannot guarantee that it will work in that application, we see no reason to believe that it wouldn't. We would of course recommend thoroughly blocking your samples to minimize any non-specific binding in a sandard ICC blocking solution containing BSA or a 10% rabbit serum.

I hope this information helps. Please let me know if you have any questions.

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Application
Flow Cytometry
This secondary antibody was used to detect Slc4a1 on mouse erythrocytes as control as well as differentiated mouse embryonic stem cells. It can be seen in the picture attached that the labelling detected with this secondary antibody alone is practically identical to that obtained with un-labelled cells, meaning that the background is very low, which increases the confidence in the specificity of the primary antibody used.

Alena Pance

Verified customer

Submitted Sep 08 2014

Question
Answer

Thank you for contacting us today regarding secondary antibodies.

I have made a short list of compatible antibodies for use withab56215 Anti-SLC4A4 antibody.These products are pre-absorbed to ensure minimum cross reactivity. Each product is conjugated a different fluorochome so that you may chose the one which best fits your experiments. Please let me know if you have any questions.

AMCA:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-AMCA-ab123435.html

PE:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-PE-pre-adsorbed-ab72465.html

DyLight 594:https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-594-pre-adsorbed-ab98500.html

Texas Red:https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-Pre-Adsorbed-ab7088.html

APC:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-Allophycocyanin-pre-adsorbed-ab130805.html

If testing live cells we also recommend cell viability controls such as 7-AAD orPropidium Iodide.Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. We do carry PI and offer a 7-AADCell-Mediated Cytotoxicity Assay Kit.

Propidium Iodide: https://www.abcam.com/Propidium-Iodide-ab14083.html

7-AAD Kit:https://www.abcam.com/7-AADCFSE-Cytotoxicity-Assay-Kit-ab133073.html



In reviewing the datasheet for this product I did note that it has not been tested in Flow Cytometry. As such we cannot guarantee ab56212 in that application. However by participating in our AbTrial program, you can now use our products in an untested application or species without financial risk.



Simply follow these easy steps below to take part in our AbTrial Program:

1. Reply to this e-mail to let me know that you would like to proceed and test this product in Flow Cytometry.

2. I will email you aninactivepersonal discount code for the value of the product.

3. Purchase and test the product at regular price.

4. https://www.abcam.com/abreviews with you results noting your AbTrial discount code in the additional notes section.

5. Once the Abreview is submitted, the discount code will becomeactive.

6. Apply your discount code on your next order to receive that value off.

More information including the terms and conditions about this program may be found at: https://www.abcam.com/AbTrial

I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.

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Question
Answer

Thank you for your reply.
Below are the 2 products that I would recommend for you:
Isotype control - https://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html
Secondary - https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-PE-pre-adsorbed-ab97070.html
Both of these are covered under our Abpromise and are guaranteed to work in Flow Cytometry.
If there is anything else I can help you with, please let me know.

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Answer

Thank you for contacting Abcam.
I would be happy to help you select the best products for your experiments. To be able to assist you further, would you please let me know which applications you wish to use these products in. That way I can recommend products that have been tested and are guaranteed to work in particular applications.
I look forward to your reply.
Also, for the month of Spetember we are running a buy 4 for 3 promotion, purhcase any 3 products and get a 4th free. If you wich to take advantage of this promotion, just place the code ** in your next order.

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Answer

Thank you for your enquiry and interest in our products. In order to be able to search the compatible secondary antibodies, first  it is important to consider the isotypes of these two primary antibodies: ab9568 - mouse IgG1 kappa ab109022 - rabbit IgG1 I would advise you and your customer to use our recently developed advanced search facilities for secondary antibodies: https://www.abcam.com/index.html?pageconfig=productmap&cl=918 I have conducted some search for you and you can find some compatible antibodies: For ab9568: ab99605 - Rat monoclonal [SB77e] Secondary Antibody to Mouse IgG1 (PE), pre-adsorbed ab99912 - Goat polyclonal Secondary Antibody to Mouse IgG1 (PE) For ab109022: ab97070 - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed -Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), ab98441- Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), ab98494- Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed, ab7007- Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed, ab72465 - Goat polyclonal Secondary Antibody to Rabbit IgG (PE), pre-adsorbed   I hope this helps and if I can assist further, please do not hesitate to contact me.    

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