Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (Texas Red ®) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Full length native Rabbit IgG (purified).

  • Conjugation
    Texas Red ®. Ex: 596nm, Em: 620nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose.
  • Conjugation notes
    Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.7 moles Texas Red per mole of Goat IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Immunomicroscopy
Flow Cyt
IHC-P
IHC-Fr
ELISA
  • Application notes
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    This product has been referenced in:
    • Zhu Y  et al. MEG3 Activated by Vitamin D Inhibits Colorectal Cancer Cells Proliferation and Migration via Regulating Clusterin. EBioMedicine 30:148-157 (2018). WB . Read more (PubMed: 29628342) »
    • Ho G  et al. Molecular imaging of retinal gliosis in transgenic mice induced by kainic acid neurotoxicity. Invest Ophthalmol Vis Sci 50:2459-64 (2009). Read more (PubMed: 19151398) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Question
    Answer

    Thank you for contacting us today regarding secondary antibodies.

    I have made a short list of compatible antibodies for use withab56215 Anti-SLC4A4 antibody.These products are pre-absorbed to ensure minimum cross reactivity. Each product is conjugated a different fluorochome so that you may chose the one which best fits your experiments. Please let me know if you have any questions.

    AMCA:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-AMCA-ab123435.html

    PE:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-PE-pre-adsorbed-ab72465.html

    DyLight 594:https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-594-pre-adsorbed-ab98500.html

    Texas Red:https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-Pre-Adsorbed-ab7088.html

    APC:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-Allophycocyanin-pre-adsorbed-ab130805.html

    If testing live cells we also recommend cell viability controls such as 7-AAD orPropidium Iodide.Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. We do carry PI and offer a 7-AADCell-Mediated Cytotoxicity Assay Kit.

    Propidium Iodide: https://www.abcam.com/Propidium-Iodide-ab14083.html

    7-AAD Kit:https://www.abcam.com/7-AADCFSE-Cytotoxicity-Assay-Kit-ab133073.html



    In reviewing the datasheet for this product I did note that it has not been tested in Flow Cytometry. As such we cannot guarantee ab56212 in that application. However by participating in our AbTrial program, you can now use our products in an untested application or species without financial risk.



    Simply follow these easy steps below to take part in our AbTrial Program:

    1. Reply to this e-mail to let me know that you would like to proceed and test this product in Flow Cytometry.

    2. I will email you aninactivepersonal discount code for the value of the product.

    3. Purchase and test the product at regular price.

    4. https://www.abcam.com/abreviews with you results noting your AbTrial discount code in the additional notes section.

    5. Once the Abreview is submitted, the discount code will becomeactive.

    6. Apply your discount code on your next order to receive that value off.

    More information including the terms and conditions about this program may be found at: https://www.abcam.com/AbTrial

    I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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