Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (TRITC)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rabbit
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    Rabbit IgG, whole molecule

  • Conjugation
    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • Conjugation notes
    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

References

This product has been referenced in:
  • Zhang X  et al. Zinc finger protein 668 suppresses non-small cell lung cancer invasion and migration by downregulating Snail and upregulating E-cadherin and zonula occludens-1. Oncol Lett 15:3806-3813 (2018). Read more (PubMed: 29556277) »
  • Zhang J  et al. Paraquat initially damages cochlear support cells leading to anoikis-like hair cell death. Hear Res 364:129-141 (2018). Read more (PubMed: 29563067) »
See all 28 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Immunomicroscopy
Goat anti-Rabbit IgG H&L (TRITC) is good to label iNOS in mouse hepatocytes.

Abcam user community

Verified customer

Submitted Aug 07 2015

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1231536.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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Answer

I am sorry hear about the disappointing results. If customer have purchased the secondary antibody from Abcam (ab6718) and if the problem is due to secondary antibody then I am happy to replace it for this customer.
Please let me know the order number for secondary antibody.

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Answer

Thank you for providing details.
This is strange, if the red cells are seen even without primary antibody then the problem is either due to secondary antibody or due to protocol. I have checked the protocol and I find no problem with it other than the wash step which I assume is 3 washes of 5 minutes.
You may need to try again, following the correct ICC/IF protocol steps.
Please let me know if the results do not improve.

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Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.
I have read the details you have kindly provided and have following further questions for better understanding of the problem;
- What was the cell line used? e.g. human liver cancer cell line.
- Do you see difference in no primary control? Were the cells red in colour as seen with primary antibody?
- Do you think the problem is due to ab6718 or ab110641.
- What was the storage condition of antibody?
- Did you change any step when doing repeat experiment?
Thank you very much for your cooperation. I will look forward to hearing from you soon.

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Answer

Thank you for your reply.

I am very sorry to hear you are still experiencing problems with these products. According to the images sent it appears the secondary is still giving a lot of non specific background.

If the antibody is still under the Abpromise guarantee I will be more than happy to send you a free of charge replacement for a different Goat anti-Rb secondary.

Could you please provide your order number?

The alternatives available are listed under the next link:

https://www.abcam.com/index.html?pageconfig=searchresults&search=IgG&pt=7&sk=app&sv=3688&sn=ICC/IF&l=3&fViewMore=1

I encourage you to have a look at it and decide which one fulfils your requirements.

In regards to the primary anti-CD31, I am afraid this antibody has not been tested and validated in ICC, and therefore we cannot guarantee it will work in this application.

I would suggest trying the new secondary I will send you free of charge, and in case you are still not obtaining the expected results, we will study more carefully the protocol and different possibilities.

I then look forward for receiving your reply, and thank you for your cooperation. Have a great day.

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Answer

Thank you for contacting us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would appreciate if you could please send me the order details, so I can check there isn’t any particular issue with the lot number received and during the shipping process. It is also important for us in order to monitor our products’ quality.

It will also be very helpful to have an image of the non primary incubation to compare it to the primary + secondary image sent.

What is the isotype of the primary antibody? Has it been validated to work in ICC?

In any case, all of our products are covered by our Abpromise guarantee; which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

I look forward for receiving your reply.

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Answer

Thank you for your enquiry. 1) Ab27089 is supplier as a pre diluted product and Ab4055 is not. 2) We do not have specific feedback in relation to certain secondary Abs functioning in conjunction with our antibodies; however a list of compatible secondary Abs are cited at the base of each online data sheet for you to choose from. I would expect any non rabbit species anti rabbit IgG with a suitable conjugation to function. 3)Looking at products Ab7087 and Ab6718 the only obvious difference that I am able to spot is the different concentrations of the product. I will pass this information onto my colleague sin business development since it is possible that we have added a duplicate product in error. Should you have any further questions then please do not hesitate to get back in contact with me.

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Answer

Thank you for your enquiry. According to the datasheet this antibody is in liquid form (the buffer is: 0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2) so you do not need to reconstitute it. The unit size is 1 mg and the concentration is 2 mg/ml. If you need anything further or any help then please let us know.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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