Product nameGoat Anti-Rabbit IgG H&L (TRITC)
See all IgG secondary antibodies
Tested applicationsSuitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
Rabbit IgG, whole molecule
ConjugationTRITC. Ex: 547nm, Em: 572nm
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
Conjugation notesTetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG
Our Abpromise guarantee covers the use of ab6718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Immunomicroscopy||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent dilution.|
|ICC/IF||1/1000 - 1/5000.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||1/10000 - 1/50000.|
This product has been referenced in:
- Zhang X et al. Zinc finger protein 668 suppresses non-small cell lung cancer invasion and migration by downregulating Snail and upregulating E-cadherin and zonula occludens-1. Oncol Lett 15:3806-3813 (2018). Read more (PubMed: 29556277) »
- Zhang J et al. Paraquat initially damages cochlear support cells leading to anoikis-like hair cell death. Hear Res 364:129-141 (2018). Read more (PubMed: 29563067) »