Goat Anti-Rabbit IgG H&L (TRITC) preadsorbed (ab7087)

Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (TRITC) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Full length native Rabbit IgG (purified).

  • Conjugation
    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose.
  • Conjugation notes
    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Immunomicroscopy
Flow Cyt
IHC-P
IHC-Fr
ELISA
  • Application notes
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    ab7087 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your recent technical service inquiry. The TRITC used for our conjugations is tetramethlyrodamine-5-(and-6)-isothiocyanate (mixed isomers). Please feel free to contact me for additional assistance regarding this issue.

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    Answer

    Thank you for your enquiry. 1) Ab27089 is supplier as a pre diluted product and Ab4055 is not. 2) We do not have specific feedback in relation to certain secondary Abs functioning in conjunction with our antibodies; however a list of compatible secondary Abs are cited at the base of each online data sheet for you to choose from. I would expect any non rabbit species anti rabbit IgG with a suitable conjugation to function. 3)Looking at products Ab7087 and Ab6718 the only obvious difference that I am able to spot is the different concentrations of the product. I will pass this information onto my colleague sin business development since it is possible that we have added a duplicate product in error. Should you have any further questions then please do not hesitate to get back in contact with me.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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