Overview

  • Product name

    Goat anti-Rabbit IgG (SureLight® Allophycocyanin)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, FRETmore details
  • Minimal
    cross-reactivity


    Cow, Mouse more details
  • Immunogen

    Rabbit IgG

  • Conjugation

    SureLight® Allophycocyanin. Ex: 652nm, Em: 657nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.013% Sodium azide
    Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate, 1.71% Sucrose
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.
  • Conjugation notes

    Excitation max. l 652 nm. Emission max. l 657.5 nm. F/P 1:1.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
FRET Use at an assay dependent concentration.

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for contacting us. I can confirm that the Surelight is only the brand name; the conjuagte is APC.  Please do check our promotion for secondary antibodies; https://www.abcam.com/index.html?pageconfig=resource&rid=14706 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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Answer

Thank you for contacting Abcam. The concentration of ab37379, does vary depending on the individual batch. The concentration of the stock that we currently have is 0.01mg/ml (Lot#1166697). As for the isotype control, the isotype control antibody should match the primary antibody’s host species, isotype, and conjugation. For example, if the primary antibody is a FITC-conjugated mouse IgG1, then you will need to choose a FITC-conjugated mouse IgG1 isotype control. In this case your primary antibody is a rabbit (?), therefore you would need a rabbit isotype control. Is your primary antibody tagged? If not then ab27427 (IgG) or ab37424 (IgM) would be more appropriate. If your primary antibody is tagged, then you would need to find a rabbit isotype control with the appropriate tag. If there is anything else I can help with, please let me know.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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