Key features and details
- Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 488), pre-adsorbed
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Product nameGoat Anti-Rat IgG Fc (DyLight® 488) preadsorbed
See all IgG secondary antibodies
DescriptionGoat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 488), pre-adsorbed
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was dtected against IgA, IgM or non-immunoglobulin serum proteins. Reduced cross-reactivity to human and mouse IgG was detected.
Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cytmore details
Human, MouseTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
ConjugationDyLight® 488. Ex: 493nm, Em: 518nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
Our Abpromise guarantee covers the use of ab96971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/500.|
|ICC/IF||1/50 - 1/500.|
|Flow Cyt||1/50 - 1/200.|
Overlay histogram showing peripheral blood lymphocytes stained with ab30446 (red line). The cells were incubated with the antibody (ab30446, 1μg/1x10^6 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (Fc) at 1/250 dilution for 30 min at 4°C. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 2μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab96971 has not yet been referenced specifically in any publications.