Goat Anti-Rat IgG Fc (DyLight® 650) preadsorbed (ab96974)

Overview

  • Product name

    Goat Anti-Rat IgG Fc (DyLight® 650) preadsorbed
    See all IgG secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 650), pre-adsorbed
  • Host species

    Goat
  • Target species

    Rat
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was dtected against IgA, IgM or non-immunoglobulin serum proteins. Reduced cross-reactivity to human and mouse IgG was detected.

     

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity


    Human, Mouse more details
  • Conjugation

    DyLight® 650. Ex: 654nm, Em: 673nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    DyLight® 650 replaces DyLight® 649.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Anti-dopamine transporter (DAT) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 650), pre-adsorbed (ab96974) was used as a secondary antibody at a concentration of 1/500.

  • Immunocytochemsitry/Immunofluorescence analysis of HEK293 cells permeabilized with 0.2% Triton X-100 using ab96974 (red) at 1/500 in PBS + 2% BSA for 1 hour.

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

ab96974 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
red channel is anti-Rat Dy650, permeablize using 0.2%TritonX-100. work at dilution of 1:500, incubate in 2%BSA/PBS at RT for 1h

Ms. Ya Zhou

Verified customer

Submitted Feb 28 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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