Product nameGoat Anti-Rat IgG H&L
See all IgG secondary antibodies
SpecificityThe antibody reacts with rat immunoglobulins of all classes. Cross-reactions as determined by ELISA (see figure below): Chicken IgY, rabbit IgG and human IgG, less than 2%. Mouse IgG, less than 7%.
Tested applicationsSuitable for: ELISA, IP, WB, IHC-Pmore details
The details of the immunogen for this antibody are not available.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Concentration information loading...
Purification notesImmunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Our Abpromise guarantee covers the use of ab182018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/20000 - 1/200000.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
Cross-reactivity of the polyclonal secondary antibody ab182018 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1µg/ml (50µl/well), and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182018 was then added starting at 1 µg/ml and gradually diluted 1:4 (50µl/well), followd by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1:20,000 dilution (50µl/well), followed by incubation for 1h at RT.
For the batch tested, ab182018 showed a cross-reactivity 7% toward mouse IgG and below 2% towards rabbit IgG, human IgG and chicken IgY.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab182018 has not yet been referenced specifically in any publications.