Goat Anti-Rat IgG H&L (Alexa Fluor® 405) (ab175671)

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Overview

  • Product name

    Goat Anti-Rat IgG H&L (Alexa Fluor® 405)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rat

  • Specificity

    This antibody is specific to Rat IgG

  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 405. Ex: 402nm, Em: 421nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section. Store In the Dark.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol, PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.

We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) 
Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm 
Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm
Flow Cyt 1/2000.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) (ab175671)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) (ab175671)

    ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes, blocked with Image-iT® FX Signal Enhancer at room temperature for 30 min and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2µg/ml) overnight at +4°C. The secondary antibody (blue) was ab175671 Alexa Fluor® 405 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DRAQ5 (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) (ab175671)
    Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) (ab175671)

References

This product has been referenced in:

  • Mantri CK & St John AL Immune synapses between mast cells and ?d T cells limit viral infection. J Clin Invest 129:1094-1108 (2019). Read more (PubMed: 30561384) »
  • Peruzzotti-Jametti L  et al. Macrophage-Derived Extracellular Succinate Licenses Neural Stem Cells to Suppress Chronic Neuroinflammation. Cell Stem Cell 22:355-368.e13 (2018). Read more (PubMed: 29478844) »
See all 4 Publications for this product

Publishing research using ab175671? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

I see this antibody has been immunogen affinity purified, but not pre-adsorbed. I do not want it to cross react with rabbit IgG, would you expect it to cross react with rabbit IgG or not?

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Answer


The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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