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  1. Link

    goat-rat-igg-hl-alexa-fluor-488-ab150157.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (96)

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
  • Flow Cytometry - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
  • Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)

Key features and details

  • Goat Anti-Rat IgG H&L (Alexa Fluor® 488)
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-Fr, Flow Cyt, IHC-P, ICC/IF, ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 6nm HRP HRP polymer PE PE Texas Red ® TRITC Unconjugated Unconjugated

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Overview

  • Product name

    Goat Anti-Rat IgG H&L (Alexa Fluor® 488)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rat
  • Tested applications

    Suitable for: IHC-Fr, Flow Cyt, IHC-P, ICC/IF, ELISAmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rat
    • IgG
    • Fluorophore
    • Alexa Fluor® 488

Associated products

  • Related Products

    • Anti-CD45 antibody [YTH24.5] (ab30446)
    • Normal Goat Serum (ab7481)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
1/2000 - 1/4000.
IHC-P (1)
Use at an assay dependent concentration.
ICC/IF (2)
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
Notes
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
1/2000 - 1/4000.
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)

    ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2µg/ml) overnight at +4°C. The secondary antibody (green) was ab150157 Alexa Fluor® 488 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Flow Cytometry - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    Flow Cytometry - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    Overlay histogram showing Jurkat cells stained with ab30446 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab30446, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor® 488) (ab150157) was used at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (96)

Publishing research using ab150157? Please let us know so that we can cite the reference in this datasheet.

ab150157 has been referenced in 96 publications.

  • Yu W  et al. Bone marrow adipogenic lineage precursors promote osteoclastogenesis in bone remodeling and pathologic bone loss. J Clin Invest 131:N/A (2021). PubMed: 33206630
  • Okada S  et al. Molecular histology of spermatogenesis in the Japanese macaque monkey (Macaca fuscata). Primates 62:113-121 (2021). PubMed: 32803510
  • Lin J  et al. H(+)/Cl(-) exchange transporter 7 promotes lysosomal acidification-mediated autophagy in mouse cardiomyocytes. Mol Med Rep 23:N/A (2021). PubMed: 33495814
  • Dong J  et al. Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH. Nat Commun 12:66 (2021). PubMed: 33397952
  • Wei Y  et al. Acacetin ameliorates insulin resistance in obesity mice through regulating Treg/Th17 balance via MiR-23b-3p/NEU1 Axis. BMC Endocr Disord 21:57 (2021). PubMed: 33781239
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

CD44 (488) staining on primary rabbit cholangiocytes

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Cells were fixed in 4% Paraformaldehyde.
Permeabilization: Triton X-100
Primary antibody: Rat anti-CD44
Blocking: 5% BSA in PBS
Counterstained with DAPI
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Egi Kardia

Verified customer

Submitted Oct 16 2020

goat anti rat IgG AF488

Excellent
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Goat anti rat IgG AF488 is an nice secondary antibody. The antibody works well and reliably.
We use it in the concentration 1: 2000.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 29 2019

Indirect Immunofluorescent labeling of a mitosis-specific primary antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Asynchronous HeLa cells were paraformaldehyde-fixed, permeablized, and immunofluorescently labeled with an anti-histone H3 (phospho S10) antibody, which is expected to stain only mitotic cells. The fluorescent secondary antibody (ab150157) exhibited the correct affinity for the primary antibody with no apparent background or non-specific staining. In the "Merge" image below, the DAPI is pseudocolored red, while ab150157 is pseudocolored green.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

Submitted Oct 18 2013

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