Overview

  • Product name

    Goat Anti-Rat IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rat
  • Tested applications

    Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • Immunogen

    Rat IgG whole molecule

  • Conjugation

    Alkaline Phosphatase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 8
    Preservative: 0.1% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.014% Zinc chloride, 50% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads.
  • Conjugation notes

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Part of the AbExcel range.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/20000.
ELISA 1/15000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Images

  • All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml (Anti-Tubulin antibody [YL1/2] - Loading Control)

    All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/5000 dilution (Goat polyclonal Secondary Antibody to Rat IgG)
    Lanes 3-4 : Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/20000 dilution (Goat polyclonal Secondary Antibody to Rat IgG)
    Lanes 5-6 : Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/50000 dilution (Goat polyclonal Secondary Antibody to Rat IgG)

    Developed using the ECL technique.

    Performed under reducing conditions.

References

This product has been referenced in:

  • Stromberg LR  et al. Presentation matters: Impact of association of amphiphilic LPS with serum carrier proteins on innate immune signaling. PLoS One 13:e0198531 (2018). Read more (PubMed: 29902192) »
  • Tarver MR  et al. Myosin Gene Expression and Protein Abundance in Different Castes of the Formosan Subterranean Termite (Coptotermes formosanus). Insects 3:1190-9 (2012). Read more (PubMed: 26466734) »
See all 2 Publications for this product

Customer reviews and Q&As

Question
Answer

Thank you for your inquiry.

I heard back from the supplying lab that this secondary will most likely cross-react with mouse IgG, due to similarity with ratIgG,although this hasn't been specifically tested.

I know you said that this was a concern with some of your other mouse IgG primary antibodies with dual staining. I'd recommend using a pre-adsorbed antibody like donkey polyclonal Secondary Antibody to Rat IgG - H&L (AP), pre-adsorbed, ab102215, which will show minimal cross-reactivity with human and mouse.
https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-AP-pre-adsorbed-ab102215.html
Or ab102249, Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (AP), pre-adsorbed with minimal cross-reactivity with Cow, Horse, Human, Mouse, Rabbit, and Sheep.
https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-AP-pre-adsorbed-ab102249.html
I hope this information helps. Please contact us with any other questions.

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