Product nameGoat Anti-Rat IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies
Tested applicationsSuitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
Rat IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 8
Preservative: 0.1% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.014% Zinc chloride, 50% Glycerol
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads.
Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
General notesPart of the AbExcel range.
Our Abpromise guarantee covers the use of ab6846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/20000.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|ICC/IF||1/200 - 1/1000.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane.
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (AP) (ab6846) at 1/5000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (AP) (ab6846) at 1/20000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (AP) (ab6846) at 1/50000 dilution
Developed using the ECL technique
Performed under reducing conditions.Predicted band size : 50 kDa
Observed band size : 52 kDaAdditional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
Abcam recommends blocking with 3% milk to reproduce this western blot data.
This product has been referenced in:
- Stromberg LR et al. Presentation matters: Impact of association of amphiphilic LPS with serum carrier proteins on innate immune signaling. PLoS One 13:e0198531 (2018). Read more (PubMed: 29902192) »
- Tarver MR et al. Myosin Gene Expression and Protein Abundance in Different Castes of the Formosan Subterranean Termite (Coptotermes formosanus). Insects 3:1190-9 (2012). Read more (PubMed: 26466734) »