Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846)
Key features and details
- Goat Anti-Rat IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr
Related conjugates and formulations
Overview
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Product name
Goat Anti-Rat IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Tested applications
Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details -
Immunogen
Rat IgG whole molecule
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.001% Zinc chloride, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads. -
Conjugation notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Part of the AbExcel range. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/20000.
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ELISA |
1/15000.
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Immunomicroscopy |
Use at an assay dependent concentration.
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|
Dot blot |
Use at an assay dependent concentration.
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|
ICC/IF |
1/200 - 1/1000.
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|
IHC-P |
Use at an assay dependent concentration.
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|
IHC-Fr |
Use at an assay dependent concentration.
|
Notes |
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WB
1/2000 - 1/20000. |
ELISA
1/15000. |
Immunomicroscopy
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
ICC/IF
1/200 - 1/1000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Images
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All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml (Anti-Tubulin antibody [YL1/2] - Loading Control)
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/5000 dilution (Goat polyclonal Secondary Antibody to Rat IgG)
Lanes 3-4 : Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/20000 dilution (Goat polyclonal Secondary Antibody to Rat IgG)
Lanes 5-6 : Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/50000 dilution (Goat polyclonal Secondary Antibody to Rat IgG)
Developed using the ECL technique.
Performed under reducing conditions.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab6846 has been referenced in 3 publications.
- Mukai K et al. Topical estrogen application promotes cutaneous wound healing in db/db female mice with type 2 diabetes. PLoS One 17:e0264572 (2022). PubMed: 35271602
- Stromberg LR et al. Presentation matters: Impact of association of amphiphilic LPS with serum carrier proteins on innate immune signaling. PLoS One 13:e0198531 (2018). PubMed: 29902192
- Tarver MR et al. Myosin Gene Expression and Protein Abundance in Different Castes of the Formosan Subterranean Termite (Coptotermes formosanus). Insects 3:1190-9 (2012). PubMed: 26466734