Key features and details
- Goat Anti-Rat IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Product nameGoat Anti-Rat IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG and with light chains common to other Rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
Other Immunogen Type corresponding to Rat IgG.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
Conjugation notesMolar enzyme: antibody protein ratio is 1:1
Our Abpromise guarantee covers the use of ab97054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|IHC-P||1/100 - 1/1000.|
|ELISA||1/5000 - 1/50000. (Primary).|
|WB||1/5000 - 1/50000. (Chemiluminescent).|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97054 has been referenced in 1 publication.
- Giannini LAA et al. Empiric Methods to Account for Pre-analytical Variability in Digital Histopathology in Frontotemporal Lobar Degeneration. Front Neurosci 13:682 (2019). PubMed: 31333403