Goat Anti-Rat IgG H&L (Biotin) (ab6844)

Submit a review Q&A (1)References (9)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (Biotin) (ab6844)

    Key features and details

    • Goat Anti-Rat IgG H&L (Biotin)
    • Conjugation: Biotin
    • Host species: Goat
    • Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr

    Overview

    • Product name

      Goat Anti-Rat IgG H&L (Biotin)
      See all IgG secondary antibodies

    • Host species

      Goat

    • Target species

      Rat

    • Tested applications

      Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details

    • Immunogen

      Rat IgG whole molecule

    • Conjugation

      Biotin

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.88% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
    • Concentration information loading...

    • Purity

      Affinity purified

    • Purification notes

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • Conjugation notes

      Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule

    • Clonality

      Polyclonal

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6844 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use at an assay dependent dilution.
    ELISA
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent concentration.
    Dot blot
    Use at an assay dependent concentration.
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    Notes
    WB
    Use at an assay dependent dilution.
    ELISA
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent concentration.
    Dot blot
    Use at an assay dependent concentration.
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (Biotin) (ab6844)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (Biotin) (ab6844)
      Ab6844 was used at dilution 1/500 with the primary antibody ab6640 in IHC-P. See the review on ab6640.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (9)

    Publishing research using ab6844? Please let us know so that we can cite the reference in this datasheet.

    ab6844 has been referenced in 9 publications.

    • Casagrande V  et al. Restoration of renal TIMP3 levels via genetics and pharmacological approach prevents experimental diabetic nephropathy. Clin Transl Med 11:e305 (2021). PubMed: 33634991
    • Zoetemelk M  et al. Optimized low-dose combinatorial drug treatment boosts selectivity and efficacy of colorectal carcinoma treatment. Mol Oncol 14:2894-2919 (2020). PubMed: 33021054
    • Chen D  et al. MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer. Oncol Rep 42:679-687 (2019). PubMed: 31233194
    • Seminerio MJ  et al. The evaluation of AZ66, an optimized sigma receptor antagonist, against methamphetamine-induced dopaminergic neurotoxicity and memory impairment in mice. Int J Neuropsychopharmacol 16:1033-44 (2013). PubMed: 22932447
    • Kaushal N  et al. Pharmacological evaluation of SN79, a sigma (s) receptor ligand, against methamphetamine-induced neurotoxicity in vivo. Eur Neuropsychopharmacol 23:960-71 (2013). PubMed: 22921523
    • Sanchez-Adams J  et al. Atomic force microscopy reveals regional variations in the micromechanical properties of the pericellular and extracellular matrices of the meniscus. J Orthop Res 31:1218-25 (2013). PubMed: 23568545
    • Tachibana H  et al. Activation of liver X receptor inhibits osteopontin and ameliorates diabetic nephropathy. J Am Soc Nephrol 23:1835-46 (2012). PubMed: 23085633
    • Cicko S  et al. Purinergic receptor inhibition prevents the development of smoke-induced lung injury and emphysema. J Immunol 185:688-97 (2010). IHC-P . PubMed: 20519655
    • Martorana PA  et al. Effect of roflumilast on inflammatory cells in the lungs of cigarette smoke-exposed mice. BMC Pulm Med 8:17 (2008). IHC-P . PubMed: 18755021

    Customer reviews and Q&As

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    Question

    I would like to use this antibody for triple immuno-labelling with mouse, rat and rabbit primaries. Has this secondary been adsorbed against mouse IgG/serum? Also, the conjugation notes say "10-20 BAC molecules per Rabbit IgG molecule". Surely, that should say goat IgG?

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    Answer

    Thank you for your equiry. This antibody has not been adsorbed against mouse. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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