Goat Anti-Rat IgG H&L (Biotin) (ab6844)

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Overview

  • Product name
    Goat Anti-Rat IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rat
  • Tested applications
    Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • Immunogen

    Rat IgG whole molecule

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (Biotin) (ab6844)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (Biotin) (ab6844)
    Ab6844 was used at dilution 1/500 with the primary antibody ab6640 in IHC-P. See the review on ab6640.

References

This product has been referenced in:
  • Kaushal N  et al. Pharmacological evaluation of SN79, a sigma (s) receptor ligand, against methamphetamine-induced neurotoxicity in vivo. Eur Neuropsychopharmacol 23:960-71 (2013). Read more (PubMed: 22921523) »
  • Sanchez-Adams J  et al. Atomic force microscopy reveals regional variations in the micromechanical properties of the pericellular and extracellular matrices of the meniscus. J Orthop Res 31:1218-25 (2013). Read more (PubMed: 23568545) »
See all 5 Publications for this product

Publishing research using ab6844? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

I would like to use this antibody for triple immuno-labelling with mouse, rat and rabbit primaries. Has this secondary been adsorbed against mouse IgG/serum? Also, the conjugation notes say "10-20 BAC molecules per Rabbit IgG molecule". Surely, that should say goat IgG?

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Answer

Thank you for your equiry. This antibody has not been adsorbed against mouse. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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