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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rat IgG H&L (Biotin) (ab6844)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (7)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (Biotin) (ab6844)

    Key features and details

    • Goat Anti-Rat IgG H&L (Biotin)
    • Conjugation: Biotin
    • Host species: Goat
    • Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr

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    Overview

    • Product name

      Goat Anti-Rat IgG H&L (Biotin)
      See all IgG secondary antibodies
    • Host species

      Goat
    • Target species

      Rat
    • Tested applications

      Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
    • Immunogen

      Rat IgG whole molecule

    • Conjugation

      Biotin

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.88% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
    • Concentration information loading...
    • Purity

      Affinity purified
    • Purification notes

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    • Conjugation notes

      Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
    • Clonality

      Polyclonal
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Rat
      • IgG
      • Biotin

    Associated products

      Applications

      Our Abpromise guarantee covers the use of ab6844 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      WB Use at an assay dependent dilution.
      ELISA Use at an assay dependent dilution.
      Immunomicroscopy Use at an assay dependent concentration.
      Dot blot Use at an assay dependent concentration.
      ICC/IF 1/1000 - 1/5000.
      IHC-P Use at an assay dependent concentration.
      IHC-Fr Use at an assay dependent concentration.

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (Biotin) (ab6844)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (Biotin) (ab6844)
        Ab6844 was used at dilution 1/500 with the primary antibody ab6640 in IHC-P. See the review on ab6640.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (7)

      Publishing research using ab6844? Please let us know so that we can cite the reference in this datasheet.

      ab6844 has been referenced in 7 publications.

      • Chen D  et al. MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer. Oncol Rep 42:679-687 (2019). PubMed: 31233194
      • Seminerio MJ  et al. The evaluation of AZ66, an optimized sigma receptor antagonist, against methamphetamine-induced dopaminergic neurotoxicity and memory impairment in mice. Int J Neuropsychopharmacol 16:1033-44 (2013). PubMed: 22932447
      • Kaushal N  et al. Pharmacological evaluation of SN79, a sigma (s) receptor ligand, against methamphetamine-induced neurotoxicity in vivo. Eur Neuropsychopharmacol 23:960-71 (2013). PubMed: 22921523
      • Sanchez-Adams J  et al. Atomic force microscopy reveals regional variations in the micromechanical properties of the pericellular and extracellular matrices of the meniscus. J Orthop Res 31:1218-25 (2013). PubMed: 23568545
      • Tachibana H  et al. Activation of liver X receptor inhibits osteopontin and ameliorates diabetic nephropathy. J Am Soc Nephrol 23:1835-46 (2012). PubMed: 23085633
      • Cicko S  et al. Purinergic receptor inhibition prevents the development of smoke-induced lung injury and emphysema. J Immunol 185:688-97 (2010). IHC-P . PubMed: 20519655
      • Martorana PA  et al. Effect of roflumilast on inflammatory cells in the lungs of cigarette smoke-exposed mice. BMC Pulm Med 8:17 (2008). IHC-P . PubMed: 18755021

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      I would like to use this antibody for triple immuno-labelling with mouse, rat and rabbit primaries. Has this secondary been adsorbed against mouse IgG/serum? Also, the conjugation notes say "10-20 BAC molecules per Rabbit IgG molecule". Surely, that should say goat IgG?

      Read More

      Abcam community

      Verified customer

      Asked on Nov 23 2005

      Answer

      Thank you for your equiry. This antibody has not been adsorbed against mouse. I hope this information helps. Please do not hesitate to contact us if you need anything further.

      Read More

      Abcam Scientific Support

      Answered on Nov 23 2005

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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