Overview

  • Product name

    Goat Anti-Rat IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rat
  • Tested applications

    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details
  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000 - 1/400000. (Primary).
WB 1/20000 - 1/400000. (Chemiluminescent).
IHC-P 1/500 - 1/5000.
ICC/IF 1/200 - 1/1000.

References

ab98414 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us. I am very glad to hear that you will be using our products in your experiments. I can gladly recommend three biotin labeled antibodies to suit your needs and have placed links for biotin labeled antibodies below. I would like to offer a second option which will allow you to perform these experiments but will allow you future flexibility as well. I recommend purchase of unlabeled secondary antibodies and labeling these with the marker of your choice (biotin, fluorochrome) using our EasyLink direct conjugation kits. In this way you will be able to perform serial stains using a single primary/secondary combination and will be able to optimize and trust them in this assay. You will also have the ability to use these same antibodies, apply different labels and use them in different assays such as a double or triple staining with fluorescent tags, western blot with HRP or other assays. Our EasyLink antibody conjugation kits provide asimple and quick linking process whichis part of a new generation of conjugation procedures. These eliminate many of the difficulties which were common in previous generation of conjugation kits andallow a quick and easy process to conjugate an antibody of your choice, in whatever function you need. It has the advantage of being aone-stepantibody labeling method withno separation steps. Further information about our EasyLink kits may be found at the following links: Landing Page:https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink FAQs:https://www.abcam.com/index.html?pageconfig=resource&rid=13156 The unconjugated antibodies which I would recommend would be: ab98800, anti-mouse, unconjugated:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-pre-adsorbed-ab98800.html ab98383, anti-rat, unconjugated:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-pre-adsorbed-ab98383.html ab5738, anti-Armenian hamster, unconjugated:https://www.abcam.com/Armenian-Hamster-IgG-secondary-antibody-H-L-ab5738.html For your initial biotin labeling I would recommend one of the following EasyLink Biotin (type A) labeling kits: 3 x10ug size:https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-10%C2%B5g-Biotin-ab102865.html 3 x 100ug size:https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100%C2%B5g-Biotin-ab102864.html The pre-conjugated biotin labeled antibodies can be found at the following links: ab98802 anti-mouse, biotin:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Biotin-pre-adsorbed-ab98802.html ab98414 anti-rat, biotin:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-Biotin-pre-adsorbed-ab98414.html ab5744 anti-Armenian hamster, biotin:https://www.abcam.com/Armenian-Hamster-IgG-secondary-antibody-H-L-ab5744.html Finally, as the anti-mannose receptor antibody has not yet been tested in IHC-P, I would like to offer to you enrollment in our 100% Abreview testing discount program. DISCOUNT CODE: XXXXXXX Expiration date: XXXXX There is no obligation for you to participate in this program; however should you wish to participate this code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for IHC-P with ab8918 and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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