Goat Anti-Rat IgG H&L (Cy2 ®) preadsorbed (ab6952)
Key features and details
- Goat Anti-Rat IgG H&L (Cy2 ®) preadsorbed
- Conjugation: Cy2 ®. Ex: 489nm, Em: 505nm
- Host species: Goat
- Suitable for: ICC/IF, ICC, Flow Cyt, IHC-P, IHC-Fr, ELISA
Related conjugates and formulations
Overview
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Product name
Goat Anti-Rat IgG H&L (Cy2 ®) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Tested applications
Suitable for: ICC/IF, ICC, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details -
Immunogen
Full length native Rat IgG (purified).
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Conjugation
Cy2 ®. Ex: 489nm, Em: 505nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive -
Conjugation notes
Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 2.8 moles Cy2 per mole of Goat IgG -
Clonality
Polyclonal -
General notes
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000 - 1/5000.
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ICC |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent dilution.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ELISA |
1/10000 - 1/50000.
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Notes |
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ICC/IF
1/1000 - 1/5000. |
ICC
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent dilution. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ELISA
1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab6952 has been referenced in 3 publications.
- Chen L et al. Hyperspectral scanning laser optical tomography. J Biophotonics 12:e201800221 (2019). PubMed: 30187691
- Tatematsu Y et al. Thrombospondin-1 Is Necessary for the Development and Repair of Corneal Nerves. Int J Mol Sci 19:N/A (2018). PubMed: 30332778
- Anastasiades PG et al. GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex. Nat Commun 7:10584 (2016). IHC-FoFr . PubMed: 26843463