Overview

  • Product name

    Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rat
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details
  • Conjugation

    Cy5 ®. Ex: 650nm, Em: 667nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy5.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/100 - 1/1000.

Images

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rat anti-perlecan alpha 1, 1:100
    Secondary antibody:Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5®) (ab98418), 1:100
    Nuclei were counterstained with SYTO 16

References

ab98418 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Yes, thank you, that does answer my question. I misunderstood how intended to use the antibody. If you are using this as a secondary antibody, rather than a primary, it is covered by our guarantee, even though IHC on frozen sections is not listed as a tested application. Since it is covered by our guarantee, it is not eligible for the testing discount program.

We still encourage you to send a review of your results via our Abreview program, which will earn you credits towards a future purchase, but not 100% of the cost. Here is a link with more details, if you are not familiar with reviewing our products:

www.abcam.com/abreviews

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Answer

Thank you for contacting us.

We do have a testing discount program but this anti-rat IgG antibody has been purified to remove cross-reactivity with mouse IgG. Have you considered any of the secondary antibodies that are raised against mouse IgG?

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