Product nameGoat Anti-Rat IgG H&L (Cy5 ®) preadsorbed
See all IgG secondary antibodies
Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cytmore details
Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
ConjugationCy5 ®. Ex: 650nm, Em: 667nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy5.
Our Abpromise guarantee covers the use of ab98418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/500.|
|ICC/IF||1/50 - 1/500.|
|Flow Cyt||1/100 - 1/1000.|
Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
- Fix in 3%PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 µm thickness
Primary antibody: Rat anti-perlecan alpha 1, 1:100
Secondary antibody:Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5®) (ab98418), 1:100
Nuclei were counterstained with SYTO 16
ab98418 has not yet been referenced specifically in any publications.