Overview

  • Product name
    Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5 ®), pre-adsorbed
  • Host species
    Goat
  • Target species
    Rat
  • Tested applications
    Suitable for: ICC, Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Frmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details
  • Immunogen

    Rat IgG, whole molecule (H&L)

  • Conjugation
    Cy5 ®. Ex: 650nm, Em: 667nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
ELISA 1/10000 - 1/50000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Images

  • Ab6565 was used at dilution 1/500 with the primary antibody ab6161 in ICC/IF. See the review on ab6161.

References

This product has been referenced in:
  • Kuo WY  et al. STAT3/NF-?B-Regulated Lentiviral TK/GCV Suicide Gene Therapy for Cisplatin-Resistant Triple-Negative Breast Cancer. Theranostics 7:647-663 (2017). Read more (PubMed: 28255357) »
  • Iyer DR & Rhind N Replication fork slowing and stalling are distinct, checkpoint-independent consequences of replicating damaged DNA. PLoS Genet 13:e1006958 (2017). Read more (PubMed: 28806726) »
See all 8 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you very much for your interest in our products. I can confirm that ab6346 is tested and guaranteed for ELISA whereas ab6565 is not yet tested. Please find a testing discount offer below for ab6565. I attached protocols for indirect and direct ELISA for the information of your customer. These protocols and more can also be found using this link: https://www.abcam.com/index.html?pageconfig=popular_protocols   I hope this information is helpful. Please do not hesitate to contact me again with any further questions. If you do, could you please include more information about the planned experiments of your customer? This will make it easier for me to help your customer with more specific suggestions.   Testing Discount Offer: To our knowledge, ab6565 has not been tested in ELISA. Therefore, I can offer a discount off a future purchase if you buy ab6565 now, test it in ELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free secondary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab6565 in ELISA. I will then send a discount code. This code must be issued before purchasing ab6565 so please wait for my reply before ordering. 2. Purchase ab6565 either by phone, fax, or online (www.abcam.com). 3. Test it in ELISA. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any secondary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab6565 turns out to be unsuitable for ELISA, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

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Answer

Thank you for your response and for clarifying the cell types used in the experiments.    I understand now that bEnd3 cells (immortalized mouse brain endothelial cell line) were stained for CD31. The protocol looks absolutely fine and it you have an alternative antibody which worked fine with these cells. I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.   I could raise either a credit note for the cost of  one vial of ab7388 and ab7388 since you have alternative antibodies; or send you two replacement vials (one of each)  free of charge. Please could you let me know how you wish to proceed with your enquiry? I look forward to hearing from you soon.

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Answer

Thank you for your response. Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem. 1)  Sample: Could you specify the samples used for the staining i.e. species, cells (cultured) or tissues? 2) Detection system: Have you used the secondary antibody successfully with another primary antibody? Do you think that the problem may come from the secondary antibody? Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

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Answer

Thank you for your enquiry. I can confirm that ab6565 Goat polyclonal Secondary Antibody to Rat IgG has been cross adsorbed against mouse serum proteins and has less than 1% cross reactivity against mouse polyclonal IgG by ELISA testing. I hope this information will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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Question
Answer

Thank you for your enquiry. The concentration of this antibody is 1mg/ml. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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