Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed (ab6565)


  • Product name
    Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5 ®), pre-adsorbed
  • Host species
  • Target species
  • Tested applications
    Suitable for: ICC, Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Frmore details
  • Minimal

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details
  • Immunogen

    Rat IgG, whole molecule (H&L)

  • Conjugation
    Cy5 ®. Ex: 650nm, Em: 667nm


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG
  • Clonality
  • Isotype
  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.

    This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research areas


Our Abpromise guarantee covers the use of ab6565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
ELISA 1/10000 - 1/50000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.


  • Ab6565 was used at dilution 1/500 with the primary antibody ab6161 in ICC/IF. See the review on ab6161.


This product has been referenced in:
  • Kuo WY  et al. STAT3/NF-?B-Regulated Lentiviral TK/GCV Suicide Gene Therapy for Cisplatin-Resistant Triple-Negative Breast Cancer. Theranostics 7:647-663 (2017). Read more (PubMed: 28255357) »
  • Iyer DR & Rhind N Replication fork slowing and stalling are distinct, checkpoint-independent consequences of replicating damaged DNA. PLoS Genet 13:e1006958 (2017). Read more (PubMed: 28806726) »

See all 7 Publications for this product

Customer reviews and Q&As

Thank you very much for your interest in our products. I can confirm that ab6346 is tested and guaranteed for ELISA whereas ab6565 is not yet tested. Please find a testing discount offer below for ab6565. I attached protocols for indirect and direct EL...

Read More

Thank you for your response and for clarifying the cell types used in the experiments.    I understand now that bEnd3 cells (immortalized mouse brain endothelial cell line) were stained for CD31. The protocol looks absolutely fine and it you have an...

Read More

Thank you for your response. Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem. 1)  Sample: Could you specify the samples used ...

Read More

Thank you for your enquiry. I can confirm that ab6565 Goat polyclonal Secondary Antibody to Rat IgG has been cross adsorbed against mouse serum proteins and has less than 1% cross reactivity against mouse polyclonal IgG by ELISA testing. I hope ...

Read More

Thank you for your enquiry. The concentration of this antibody is 1mg/ml. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thanks for your enquiry,unfortunately we do not supply sample sized testers. As far as I am aware both CY5 and CY 5.5 are red. If you log on to our web page and click on secondary antibodies there is a list of all the secondary antibodies we supply alo...

Read More


Sign up