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In the title of the attchament I said I used Cell cultures from bEnd3 cells. Yes, I though that the problem could be the secondary antibody but I bought it to abcam at the same time (the problem is the same) and to clarify this issue I need another primary of the same specie (rat) because I don't have it to test. Thanks in advance,
Asked on Oct 19 2011
Thank you for your response and for clarifying the cell types used in the experiments. I understand now that bEnd3 cells (immortalized mouse brain endothelial cell line) were stained for CD31. The protocol looks absolutely fine and it you have an alternative antibody which worked fine with these cells. I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased. I could raise either a credit note for the cost of one vial of ab7388 and ab7388 since you have alternative antibodies; or send you two replacement vials (one of each) free of charge. Please could you let me know how you wish to proceed with your enquiry? I look forward to hearing from you soon.
Answered on Oct 19 2011