Overview

  • Product name

    Goat Anti-Rat IgG H&L (DyLight® 550)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rat
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG and with light chains common to other Rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Conjugation

    DyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    DyLight® 550 replaces DyLight® 549.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:

  • Chesarino NM  et al. E3 Ubiquitin Ligase NEDD4 Promotes Influenza Virus Infection by Decreasing Levels of the Antiviral Protein IFITM3. PLoS Pathog 11:e1005095 (2015). IHC-P . Read more (PubMed: 26263374) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply. I hope that we may find the right product for you. We offer a large number of secondary antibodies against rabbit IgG. I see that you already have experience with our Dylight series of fluorescent secondaries. Since I do not know the excitation/emmision point tha you plan to use I cannot point to a specific product. However, my recommendations when choosing one are that you choose one raised in goat. In this way you need not be concerned about increased background staining as your other secondary against the rat primary is also raised in goat. Therefore using a block containing normal goat serum should work well for you. You may find these products using the search bar on the Abcam website. By typing Dylight and using the pulldown menu just to the right of the search field to select secondary antibodies.On the results page you will see a number of limitors to the left, These are labeled 'Target', 'Application', 'Host', etc. Click on the one labeled 'Target Species'. A short list will drop down. At the bottom of that list will be a link to 'View More'. If you click on that a pop-up window will appear that allows you to choose the species of interest, in this case rabbit. You may further limit that search by choosing theemmision/excitation pointthat is best for you under the 'Conjugation'limitorto the left. You may do much the same to find an appropriate anti-goat secondary. For this you will just need to change the target species from rabbit to goat. You should not be concerned with cross reactivity with the anti-Rat secondary in this way. Of note however is that we cannot offer a goat raised anti-goat secondary antibody. You will need to chose an antibody raised in another species. Rabbit and donkey are common choices. I would recommend that you add the serum of the host species that your secondary is raised in to your blocking solutions to try and limit the non-specific binding of that antibody to your tissue. i would still highly recommend using one of our EasyLink conjugation kits to directly conjugate a fluorecent tag to whichever antibody you choose. This way you can avoid high background caused by non-specific secondary binding, skip the secondary antibody incubation and wash stepsand conjugated antibody can be used straight away. I hope this information has been useful, please contact me if you have any questions. More information aboutEasyLink direct conjugation kits is available here: https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink

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Answer

Thank you for contacting Abcam. The difficulty with the experiment as you have set it up is that you would need three different secondary antibodies, each of which would need to not recognize the tissue or the other primary antibodies, and in reality you have only two targets. An easy and efficent way to remedy or circumvent any issue related to secondary antibodies is the directly congujate a tag, in your case a flouescent one, to your antibody. We offer a wide range of EasyLink direct fluorescent conjugation kits that would suit your needs. I've included a link below. https://www.abcam.com/index.html?pageconfig=resource&rid=13148#1 Let me know if there is anything else that I might do for you or if you have any questions.

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