Overview

  • Product name

    Goat Anti-Rat IgG H&L (DyLight® 594)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rat
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG and with light chains common to other Rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Conjugation

    DyLight® 594. Ex: 593nm, Em: 618nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your response.

We do not sell Alexa-conjugated secondary antibodies. However, we have launched a new family of DyLight® fluorochrome- labelled secondaries which exhibit higher fluorescence intensity, photostability and water solubility and remain fluorescent from pH 4 to pH 9.

Additionally, the water solubility of the DyLight® fluorochromes allows a high dye-to-antibody ratio to be achieved without causing precipitation of conjugates. You customer can find some further useful details on DyLight® secondary antibodies at our home site:

https://www.abcam.com/index.html?pageconfig=resource&rid=12934

I can confirm that both ab96885 and ab96889 have been tested for Flow cytometry and ICC/IF. However, please check with your customer the compatibility with the primary antibody(ies) before making the final purchase decision.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up