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    goat-rat-igg-hl-fitc-ab6840.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rat IgG H&L (FITC) (ab6840)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (26)

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (FITC) (ab6840)

    Key features and details

    • Goat Anti-Rat IgG H&L (FITC)
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 Gold 10nm Gold 12nm Gold 6nm HRP HRP polymer PE PE Texas Red ® TRITC Unconjugated Unconjugated

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    Overview

    • Product name

      Goat Anti-Rat IgG H&L (FITC)
      See all IgG secondary antibodies
    • Host species

      Goat
    • Target species

      Rat
    • Tested applications

      Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
    • Immunogen

      Rat whole molecule

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
    • Concentration information loading...
    • Purity

      Affinity purified
    • Purification notes

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    • Conjugation notes

      Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Rat
      • IgG
      • Fluorophore
      • FITC

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6840 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Immunomicroscopy
    Flow Cyt
    ICC/IF
    IHC-P
    IHC-Fr
    ELISA
    Notes
    Application notes
    Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency

    Images

    • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (FITC) (ab6840)
      Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (FITC) (ab6840)
      Ab6840 was used at dilution 1/500 with the primary antibody ab6326 in ICC/IF. See the review on ab6326.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (26)

    Publishing research using ab6840? Please let us know so that we can cite the reference in this datasheet.

    ab6840 has been referenced in 26 publications.

    • Wilmowicz E  et al. EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers. Int J Mol Sci 22:N/A (2021). PubMed: 33809409
    • Meng R  et al. Generation and identification of endothelial-specific Hrh2 knockout mice. Transgenic Res 30:251-261 (2021). PubMed: 33786748
    • Wang F  et al. Accelerated Bone Regeneration by Astragaloside IV through Stimulating the Coupling of Osteogenesis and Angiogenesis. Int J Biol Sci 17:1821-1836 (2021). PubMed: 33994865
    • Chang M  et al. Genistein acts as antidepressant agent against chronic mild stress-induced depression model of rats through augmentation of brain-derived neurotrophic factor. Brain Behav 11:e2300 (2021). PubMed: 34333865
    • Chothe SK  et al. NLRC5 Serves as a Pro-viral Factor During Influenza Virus Infection in Chicken Macrophages. Front Cell Infect Microbiol 10:230 (2020). PubMed: 32509599
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Hi, Do you have any secondary antibody (IHC-Fr) that works good for ab6640 Rat F4/80? The fluorescent conjugate could be either CY2 or FITC. Thank you. Best,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 05 2011

    Answer

    Thank you for contacting us. The anti Rat secondary antibody compatible for ab6640 is CY2 conjugate: ab6952; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6952.html FITC conjugate: ab6840; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6840.html ab7093: https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab7093.html There are many other different types of secondary antibodies available in our catalogue. Please try our secondary search tool on Abcam website. You can nicely select conjugate, format and species of required ab from drop down menu. Click the link to view the search tool: https://www.abcam.com/index.html?pageconfig=productmap&cl=918 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Sep 05 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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