Overview

  • Product name
    Goat Anti-Rat IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rat
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    Rat whole molecule

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
IHC-Fr
ELISA
  • Application notes
    Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
  • Images

    • Ab6840 was used at dilution 1/500 with the primary antibody ab6326 in ICC/IF. See the review on ab6326.

    References

    This product has been referenced in:
    • Wu Y  et al. Long noncoding RNA hypoxia-inducible factor 1 alpha-antisense RNA 1 promotes tumor necrosis factor-a-induced apoptosis through caspase 3 in Kupffer cells. Medicine (Baltimore) 97:e9483 (2018). WB . Read more (PubMed: 29369172) »
    • Trevisan M  et al. Generation of a transgene-free induced pluripotent stem cells line (UNIPDi002-A) from oral mucosa epithelial stem cells carrying the R304Q mutation in TP63 gene. Stem Cell Res 28:149-152 (2018). Read more (PubMed: 29486400) »
    See all 12 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for contacting us. The anti Rat secondary antibody compatible for ab6640 is CY2 conjugate: ab6952; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6952.html FITC conjugate: ab6840; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6840.html ab7093: https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab7093.html There are many other different types of secondary antibodies available in our catalogue. Please try our secondary search tool on Abcam website. You can nicely select conjugate, format and species of required ab from drop down menu. Click the link to view the search tool: https://www.abcam.com/index.html?pageconfig=productmap&cl=918 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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