Goat Anti-Rat IgG H&L (FITC) (ab6840)

Submit a review Q&A (1)References (15)

Overview

  • Product name

    Goat Anti-Rat IgG H&L (FITC)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rat

  • Tested applications

    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details

  • Immunogen

    Rat whole molecule

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
IHC-Fr
ELISA
  • Application notes
    Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency

    • Images

    • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (FITC) (ab6840)
      Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (FITC) (ab6840)
      Ab6840 was used at dilution 1/500 with the primary antibody ab6326 in ICC/IF. See the review on ab6326.

    References

    This product has been referenced in:

    • Shan L  et al. Fangchinoline supplementation attenuates inflammatory markers in experimental rheumatoid arthritis-induced rats. Biomed Pharmacother 111:142-150 (2019). Read more (PubMed: 30579253) »
    • Ren SX  et al. Mechanistic evaluation of anti-arthritic activity of ß-methylphenylalanine in experimental rats. Biomed Pharmacother 113:108730 (2019). Read more (PubMed: 30861411) »
    See all 15 Publications for this product

    Publishing research using ab6840? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Hi, Do you have any secondary antibody (IHC-Fr) that works good for ab6640 Rat F4/80? The fluorescent conjugate could be either CY2 or FITC. Thank you. Best,

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    Answer

    Thank you for contacting us. The anti Rat secondary antibody compatible for ab6640 is CY2 conjugate: ab6952; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6952.html FITC conjugate: ab6840; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6840.html ab7093: https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab7093.html There are many other different types of secondary antibodies available in our catalogue. Please try our secondary search tool on Abcam website. You can nicely select conjugate, format and species of required ab from drop down menu. Click the link to view the search tool: https://www.abcam.com/index.html?pageconfig=productmap&cl=918 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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