Goat Anti-Rat IgG H&L (FITC) (ab6840)

Submit a review Q&A (1)References (20)
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (FITC) (ab6840)

    Key features and details

    • Goat Anti-Rat IgG H&L (FITC)
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

    Conjugates logo Related conjugates and formulations

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    Overview

    • Product name

      Goat Anti-Rat IgG H&L (FITC)
      See all IgG secondary antibodies

    • Host species

      Goat

    • Target species

      Rat

    • Tested applications

      Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details

    • Immunogen

      Rat whole molecule

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
    • Concentration information loading...

    • Purity

      Affinity purified

    • Purification notes

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • Conjugation notes

      Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6840 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Immunomicroscopy
    Flow Cyt
    ICC/IF
    IHC-P
    IHC-Fr
    ELISA
    Notes
    Application notes
    Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency

    Images

    • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (FITC) (ab6840)
      Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (FITC) (ab6840)
      Ab6840 was used at dilution 1/500 with the primary antibody ab6326 in ICC/IF. See the review on ab6326.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (20)

    Publishing research using ab6840? Please let us know so that we can cite the reference in this datasheet.

    ab6840 has been referenced in 20 publications.

    • De Storme N & Geelen D High temperatures alter cross-over distribution and induce male meiotic restitution in Arabidopsis thaliana. Commun Biol 3:187 (2020). PubMed: 32327690
    • Chothe SK  et al. NLRC5 Serves as a Pro-viral Factor During Influenza Virus Infection in Chicken Macrophages. Front Cell Infect Microbiol 10:230 (2020). PubMed: 32509599
    • Zheng T  et al. Cornel iridoid glycoside exerts a neuroprotective effect on neuroinflammation in rats with brain injury by inhibiting NF-?B and STAT3. 3 Biotech 9:195 (2019). PubMed: 31065495
    • Li H  et al. Neuroprotective effects of increasing levels of HSP70 against neuroinflammation in Parkinson's disease model by inhibition of NF-?B and STAT3. Life Sci 234:116747 (2019). PubMed: 31408661
    • Ouyang Z  et al. l-Cysteine augments microtubule-associated protein 2 levels and enhances antioxidant activity in rats following traumatic brain injury. 3 Biotech 9:280 (2019). PubMed: 31245244
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Hi, Do you have any secondary antibody (IHC-Fr) that works good for ab6640 Rat F4/80? The fluorescent conjugate could be either CY2 or FITC. Thank you. Best,

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    Answer

    Thank you for contacting us. The anti Rat secondary antibody compatible for ab6640 is CY2 conjugate: ab6952; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6952.html FITC conjugate: ab6840; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6840.html ab7093: https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab7093.html There are many other different types of secondary antibodies available in our catalogue. Please try our secondary search tool on Abcam website. You can nicely select conjugate, format and species of required ab from drop down menu. Click the link to view the search tool: https://www.abcam.com/index.html?pageconfig=productmap&cl=918 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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