Goat Anti-Rat IgG H&L (FITC) preadsorbed (ab7093)

Submit an Abreview Q&A (2)

Overview

  • Product name
    Goat Anti-Rat IgG H&L (FITC) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rat
  • Tested applications
    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details
  • Immunogen

    Full length native Rat IgG (purified).

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Immunomicroscopy
Flow Cyt
IHC-P
IHC-Fr
ELISA
  • Application notes
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References

    ab7093 has not yet been referenced specifically in any publications.

    Publishing research using ab7093? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

    10
    Votes: Highest First

    The host species of the primary antibody is rat, not rabbit. Also, I will be staining PFA fixed mouse brain. Does that change your recommendation?

    Thank you for your reply.

    The secondary antibody I recommended, ab7093, will react with primary antibodies raised in rat. It has also been prepared so that it will not react with antibodies or samples from rabbit or mouse. This is desirable f...

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    Hi, Do you have any secondary antibody (IHC-Fr) that works good for ab6640 Rat F4/80? The fluorescent conjugate could be either CY2 or FITC. Thank you. Best,

    Thank you for contacting us. The anti Rat secondary antibody compatible for ab6640 is CY2 conjugate: ab6952; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6952.html FITC conjugate: ab6840; https://www.abcam.com/Rat-IgG-s...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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