Goat Anti-Rat IgG H&L (FITC) preadsorbed (ab7093)

Submit a review Q&A (2)References (1)

Key features and details

  • Goat Anti-Rat IgG H&L (FITC) preadsorbed
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Goat
  • Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA

Overview

  • Product name

    Goat Anti-Rat IgG H&L (FITC) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rat

  • Tested applications

    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details

  • Immunogen

    Full length native Rat IgG (purified).

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG

  • Clonality

    Polyclonal

  • Research areas

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab7093 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    Immunomicroscopy
    Flow Cyt
    IHC-P
    IHC-Fr
    ELISA
    Notes
  • Application notes
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab7093? Please let us know so that we can cite the reference in this datasheet.

    ab7093 has been referenced in 1 publication.

    • Barney LE  et al. Tumor cell-organized fibronectin maintenance of a dormant breast cancer population. Sci Adv 6:eaaz4157 (2020). PubMed: 32195352

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    The host species of the primary antibody is rat, not rabbit. Also, I will be staining PFA fixed mouse brain. Does that change your recommendation?

    Read More
    Answer

    Thank you for your reply.

    The secondary antibody I recommended, ab7093, will react with primary antibodies raised in rat. It has also been prepared so that it will not react with antibodies or samples from rabbit or mouse. This is desirable for your experiment because it will help to ensure specificity and reduce background staining. The antibody will be fine for PFA fixed tissue that is either frozen or paraffin embedded. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Question

    Hi, Do you have any secondary antibody (IHC-Fr) that works good for ab6640 Rat F4/80? The fluorescent conjugate could be either CY2 or FITC. Thank you. Best,

    Read More
    Answer

    Thank you for contacting us. The anti Rat secondary antibody compatible for ab6640 is CY2 conjugate: ab6952; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6952.html FITC conjugate: ab6840; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6840.html ab7093: https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab7093.html There are many other different types of secondary antibodies available in our catalogue. Please try our secondary search tool on Abcam website. You can nicely select conjugate, format and species of required ab from drop down menu. Click the link to view the search tool: https://www.abcam.com/index.html?pageconfig=productmap&cl=918 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com