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  1. Link

    goat-rat-igg-hl-hrp-ab205720.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rat IgG H&L (HRP) (ab205720)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (7)

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Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

Key features and details

  • Goat Anti-Rat IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: WB, IP, ELISA, IHC-P

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 6nm HRP polymer PE PE Texas Red ® TRITC Unconjugated Unconjugated

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Overview

  • Product name

    Goat Anti-Rat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rat
  • Specificity

    The antibody used for conjugation reacts with rat immunoglobulins of all classes. Cross-reactions as determined by ELISA for the unconjugated antibody (ab182018): Chicken IgY, rabbit IgG and human IgG, less than 2%. Mouse IgG, less than 7%.
  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-Pmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rat
    • IgG
    • Enzyme
    • HRP

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab205720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/2000 - 1/20000.
IP
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-P
1/1000 - 1/10000.
Notes
WB
1/2000 - 1/20000.
IP
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-P
1/1000 - 1/10000.

Images

  • Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)
    Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)
    All lanes : Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161) at 1 µg/ml

    Lane 1 : Liver (Human) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 54 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab6161 overnight at 4°C. Antibody binding was detected using ab205720, and visualised using ECL development solution ab133406.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP) (ab205720)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP) (ab205720)

    IHC image of tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab6160 at 2ug/ml dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature.

    An HRP-conjugated secondary (Ab205720, 1/2000 dilution) was used for 1hr at room temperature.

    The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)
    Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)
    All lanes : No Primary Antibody

    Lane 1 : Liver (Human) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/2000 dilution

    Performed under reducing conditions.

    Exposure time: 15 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was incubated overnight with 2% Bovine Serum Albumin at 4°C. Any non-specific background binding was assessed by incubating the membrane with only the secondary antibody (ab205720), and visualised using ECL development solution ab133406.

  • Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)
    Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)
    All lanes : Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161) at 1 µg/ml

    Lane 1 : Liver (Human) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : ab205720 (Left Image) at 1/5000 and a competitor secondary (Right Image) at 1/5000. Notice the increased background of the competitor product.

    Performed under reducing conditions.

    Observed band size: 54 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab6161 overnight at 4°C. Antibody binding was detected using ab205720 (Left Image) and a competitor secondary (Right Image), and visualised using ECL development solution ab133406.

  • Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)
    Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)
    All lanes : No Primary Antibody

    Lane 1 : Liver (Human) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : ab205720 (Left Image) 1/2000 and a competitor secondary (Right Image) 1/2000. Notice the increased background of the competitor product.

    Performed under reducing conditions.

    Exposure time: 15 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was incubated overnight with 2% Bovine Serum Albumin at 4°C. Any non-specific background binding was assessed by incubating the membrane with ab205720 (Left Image) and a competitor secondary (Right Image), and visualised using ECL development solution ab133406.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (7)

Publishing research using ab205720? Please let us know so that we can cite the reference in this datasheet.

ab205720 has been referenced in 7 publications.

  • Lin H  et al. Nerve growth factor regulates liver cancer cell polarity and motility. Mol Med Rep 23:N/A (2021). PubMed: 33649819
  • Stewart TA  et al. Mammary mechanobiology - investigating roles for mechanically activated ion channels in lactation and involution. J Cell Sci 134:N/A (2021). PubMed: 33262312
  • Nicolau M  et al. The plant mobile domain proteins MAIN and MAIL1 interact with the phosphatase PP7L to regulate gene expression and silence transposable elements in Arabidopsis thaliana. PLoS Genet 16:e1008324 (2020). PubMed: 32287271
  • Tilburg J  et al. SLC44A2 deficient mice have a reduced response in stenosis but not in hypercoagulability driven venous thrombosis. J Thromb Haemost 18:1714-1727 (2020). PubMed: 32297475
  • Liao X  et al. Sevoflurane exerts protective effects on liver ischemia/reperfusion injury by regulating NFKB3 expression via miR-9-5p. Exp Ther Med 17:2632-2640 (2019). PubMed: 30906455
  • Wang C  et al. Cryptotanshinone Attenuates Airway Remodeling by Inhibiting Crosstalk Between Tumor Necrosis Factor-Like Weak Inducer of Apoptosis and Transforming Growth Factor Beta 1 Signaling Pathways in Asthma. Front Pharmacol 10:1338 (2019). PubMed: 31780948
  • Li QF  et al. Effects of Modest Hypoxia and Exercise on Cardiac Function, Sleep-Activity, Negative Geotaxis Behavior of Aged Female Drosophila. Front Physiol 10:1610 (2019). PubMed: 32038290

Customer reviews and Q&As

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Test ab205720 - Goat Anti-Rat IgG H&L (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Excellent specificity.
Excellent sensitivity.
WB conditions:
- Cell lisates: 20 μg protein
- non-reducing
- Primary antibody: Rat anti-Lamp1, clon 1D4B. Hybridoma Bank. Dilution 1:1000
- Secondary antibody: 1:6000
- Developer: ECL Prime, Amersham, GE Helathcare Life Sciences, Product code RPN2232
- Exposure time: 1 second, 3th film out 4
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Feb 01 2016

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