Overview

  • Product name

    Goat Anti-Rat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rat
  • Tested applications

    Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • Immunogen

    Rat IgG whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Horseradish Peroxidase (HRP)
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1500.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

References

This product has been referenced in:

  • Funk N  et al. The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4. Sci Rep 9:4515 (2019). Read more (PubMed: 30872638) »
  • Ryu I  et al. eIF4A3 Phosphorylation by CDKs Affects NMD during the Cell Cycle. Cell Rep 26:2126-2139.e9 (2019). Read more (PubMed: 30784594) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply.

The secondary ab98385 is a similar product: goat anti-rat IgG H&L conjugated to biotin and pre-adsorbed. This antibody shows minimal cross-reactivity to mouse and human IgGs. I hope this helps, please let me know if you need any additional information or assistance.

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Answer

Thank you for contacting us.

The secondary antibody ab6845 has not been pre-adsorbed or tested for reactivity with mouse IgG. I would recommend the similar antibody ab98391, which has been pre-adsorbed and shows < 1% cross-reactivity to human and mouse IgGs. I hope this helps, please let me know if you need any additional information or assistance.

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