Goat Anti-Rat IgG H&L (HRP) (ab97057)

Reviews (5)Q&A (10)References (39)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP) (ab97057)
  • Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057)

Key features and details

  • Goat Anti-Rat IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

Overview

  • Product name

    Goat Anti-Rat IgG H&L (HRP)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rat

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG and with light chains common to other Rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

  • Conjugation notes

    Molar enzyme/ antibody protein ratio is 4:1

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    Part of the AbExcel range.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (as primary)
WB 1/2000 - 1/20000. Colorimetric: Use 1/5000-1/30000, Chemiluminescent: use 1/10000-1/50000

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP) (ab97057)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP) (ab97057)

    IHC image of alpha tubulin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab64332, 5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97057, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057)
    Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057)
    All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml

    All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/2000 dilution
    Lanes 3-4 : Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/10000 dilution
    Lanes 5-6 : Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 52 kDa
    why is the actual band size different from the predicted?



     

    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (39)

Publishing research using ab97057? Please let us know so that we can cite the reference in this datasheet.

ab97057 has been referenced in 39 publications.

  • Fontana GA  et al. Rif1 S-acylation mediates DNA double-strand break repair at the inner nuclear membrane. Nat Commun 10:2535 (2019). PubMed: 31182712
  • Tang XM  et al. Thermal pretreatment promotes the protective effect of HSP70 against tendon adhesion in tendon healing by increasing HSP70 expression. Mol Med Rep 20:205-215 (2019). PubMed: 31115522
  • Liu H  et al. High hydrostatic pressure induces apoptosis of retinal ganglion cells via regulation of the NGF signalling pathway. Mol Med Rep 19:5321-5334 (2019). PubMed: 31059045
  • Hacker K  et al. A repetitive acidic region contributes to the extremely rapid degradation of the cell-context essential protein TRIM52. Sci Rep 9:7901 (2019). PubMed: 31133683
  • Zuo Z  et al. Acupuncture attenuates renal interstitial fibrosis via the TGF-ß/Smad pathway. Mol Med Rep 20:2267-2275 (2019). PubMed: 31322212
View all Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews

Good Secondary Antibody for IHC application

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
The secondary ab works well. Based on my experience for IHC the dilution factor requires optimization based on the tissue and antigen retrieval method used.
In the attached picture you can see F480 used as primary antibody and the secondary Goat Anti-Rat IgG H&L ab 97057 used at the concentration of 1:200.
The dilution 1:500 works well too.

Dr. Francesco Elia Marino

Verified customer

Submitted Apr 15 2016

Good secondary ab

 Excellent
Abreviews
abreview image
Application
Western blot
The price of this antibody is good based on the amount provided with the shipment.
I used this Antibody at the dilution 1:10000 and it worked fine in my WB.
In the attached picture ab6640 was used as primary antbody in tissue lysates from human macrophages and ab 97057 was used as secondary antibody. The secondary and primary antibodies were diluted in 2% Milk in PBS.

Dr. Francesco Elia Marino

Verified customer

Submitted Apr 15 2016

EXCELLENT ANTIBODY

 Excellent
Abreviews
Application
Western blot
We are using an 2 years old vial of this antibody, it still works well.

Abcam user community

Verified customer

Submitted Nov 05 2015

Goat anti-Rat IgG-HRP

 Excellent
Abreviews
abreview image
Application
Western blot
Antibody recognizes the Rat antibody specifically. I analyzed an HA tagged protein (0.4 OD cells equivalent) and used the anti-Rat HA antibody that was purchased from Roche and this secondary antibody ab97057 was used as secondary. I used 1:10K dilution of the antibody and incubated for 1hr at room temperature. Nitrocellulose membrane was used for blotting. On the blot the lane 1, 2, 3 were three individual clones picked after transformation. The protein of the right molecular weight was observed. (70kD).

Abcam user community

Verified customer

Submitted Mar 26 2014

Detection of E-cadherin in mouse ES cells

 Excellent
Abreviews
abreview image
Application
Western blot
Lane 1 is wild-type mouse ES cells expressing E-cadherin. Lane 2 is an Ecad-/- mES cell line. DECMA-1 primary antibody used (not Abcam). Secondary used at 1:5000 for 1 hour. Use of secondary at higher concentration of 1:2000 results in background staining.

Abcam user community

Verified customer

Submitted Nov 28 2013

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