Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)

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Key features and details

  • Goat Anti-Rat IgG H&L (HRP polymer)
  • Conjugation: HRP polymer
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: Immunoelectrophoresis, IHC-P

Overview

  • Product name

    Goat Anti-Rat IgG H&L (HRP polymer)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rat

  • Specificity

    Based on IEP, ab214882 reacts with heavy (gamma) chains on rat IgG, light chains on all rat immunoglobulins. No cross-reactivity is observed to non-immunoglobulin rat serum proteins, IgG from human or mouse.

  • Tested applications

    Suitable for: Immunoelectrophoresis, IHC-Pmore details

  • Immunogen

    Other Immunogen Type corresponding to Rat IgG. (Purified Rat IgG, whole molecule).

  • Conjugation

    HRP polymer

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.1% CMIT/MIT 3:1
    Constituents: 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    ab214882 was affinity purified using solid phase Rat IgG. Purity >95% by SDS-PAGE.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Associated products

    Applications

    Our Abpromise guarantee covers the use of ab214882 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Immunoelectrophoresis Use at an assay dependent concentration.

    This product is provided in a pre-diluted, ready-to-use format. Incubate 150 µl per slide for 1 hour.
    IHC-P Use at an assay dependent concentration.

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)

      C57BL/6 mouse spleen stained with 1 µg/ml of rat anti-mouse CD45R/B220 detected using ab214882 Goat Anti-Rat IgG H&L (HRP polymer).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab214882? Please let us know so that we can cite the reference in this datasheet.

    ab214882 has been referenced in 1 publication.

    • Nakashima H  et al. Toxicity and Efficacy of a Novel GADD34-expressing Oncolytic HSV-1 for the Treatment of Experimental Glioblastoma. Clin Cancer Res 24:2574-2584 (2018). PubMed: 29511029

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    CD31 staining in FFPE mouse kidney

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    The FFPE mouse kidney sections were deparaffinized and rehydrated. For antigen retrieval, the tissue sections were boiled in Sodium Citrate pH6 in a pressure cooker for 10 minutes. The tissue sections were blocked with 2% BSA for an hour at room temperature. Incubated with rat anti-CD31 primary antibody (1:300) overnight at 4oC. After 3 washes with TBST, the sections were incubated with goat anti-rat HRP polymer secondary antibody (ab214882) for 90 minutes at room temperature. The sections were developed in DAB after 3 washes with TBST.
    The CD31 is very distinct in the mouse kidney section.

    Abcam user community

    Verified customer

    Submitted Feb 02 2018

    Goat anti-rat IgG HRP-Polymer for IHC on frozen sections

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Frozen OCT-embedded mouse spleen:
    Section was air-dried
    Section fixed in aceton, -20C, for 10 minutes
    Peroxidase block Dako 10'
    Protein block Dako 10'
    Anti-mouse B220 (Biolegend, 0.5 mg/ml, 1:100) 30' RT
    Goat anti-rat IgG PolymerHRP 20' RT
    Colour developed with DAB
    Counterstained with haematoxylin and mounted

    Dr. Davide Lucchesi

    Verified customer

    Submitted Aug 10 2017

    Goat anti-rat IgG HRP-Polymer for IHC on paraffin sections

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Paraffin-embedded mouse spleen:
    Section was deparaffinized
    Antigen retrieval Dako, pH6 for 15' in microwave
    Peroxidase block Dako 10'
    protein block Dako 10'
    Anti-mouse B220 (Biolegend, 0.5 mg/ml, 1:100) 30' RT
    Goat anti-rat IgG PolymerHRP 20' RT
    Colour developed with DAB
    Counterstained with haematoxylin and mounted

    Dr. Davide Lucchesi

    Verified customer

    Submitted Aug 01 2017

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