Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)
Key features and details
- Goat Anti-Rat IgG H&L (HRP polymer)
- Conjugation: HRP polymer
- Host species: Goat
- Isotype: IgG
- Suitable for: Immunoelectrophoresis, IHC-P
Related conjugates and formulations
Overview
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Product name
Goat Anti-Rat IgG H&L (HRP polymer)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Specificity
Based on IEP, ab214882 reacts with heavy (gamma) chains on rat IgG, light chains on all rat immunoglobulins. No cross-reactivity is observed to non-immunoglobulin rat serum proteins, IgG from human or mouse. -
Tested applications
Suitable for: Immunoelectrophoresis, IHC-Pmore details -
Immunogen
Full length protein corresponding to Rat IgG. (Purified Rat IgG, whole molecule).
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Conjugation
HRP polymer
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Proclin 300
Constituents: 1% BSA, 98% PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
ab214882 was affinity purified using solid phase Rat IgG. Purity >95% by SDS-PAGE. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Immunoelectrophoresis |
Use at an assay dependent concentration.
This product is provided in a pre-diluted, ready-to-use format. Incubate 150 µl per slide for 1 hour. |
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| IHC-P | (2) |
Use at an assay dependent concentration.
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| Notes |
|---|
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Immunoelectrophoresis
Use at an assay dependent concentration. This product is provided in a pre-diluted, ready-to-use format. Incubate 150 µl per slide for 1 hour. |
|
IHC-P
Use at an assay dependent concentration. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)
C57BL/6 mouse spleen stained with 1 µg/ml of rat anti-mouse CD45R/B220 detected using ab214882 Goat Anti-Rat IgG H&L (HRP polymer).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab214882 has been referenced in 3 publications.
- Kireeva G et al. Modeling of Chemoperfusion vs. Intravenous Administration of Cisplatin in Wistar Rats: Adsorption and Tissue Distribution. Molecules 25:N/A (2020). PubMed: 33076418
- Li X et al. Ubiquitination of RIPK1 regulates its activation mediated by TNFR1 and TLRs signaling in distinct manners. Nat Commun 11:6364 (2020). PubMed: 33311474
- Nakashima H et al. Toxicity and Efficacy of a Novel GADD34-expressing Oncolytic HSV-1 for the Treatment of Experimental Glioblastoma. Clin Cancer Res 24:2574-2584 (2018). PubMed: 29511029