Section was deparaffinized
Antigen retrieval Dako, pH6 for 15' in microwave
Peroxidase block Dako 10'
protein block Dako 10'
Anti-mouse B220 (Biolegend, 0.5 mg/ml, 1:100) 30' RT
Goat anti-rat IgG PolymerHRP 20' RT
Colour developed with DAB
Counterstained with haematoxylin and mounted
Dr. Davide Lucchesi
Submitted Aug 01 2017